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Literature summary extracted from

  • Zabel, P.; Dorssers, L.; Wernars, K.; van Kammen, A.
    Terminal uridylyl transferase of Vigna unguiculata: purification and characterization of an enzyme catalyzing the addition of a single UMP residue to the 3-end of an RNA primer (1981), Nucleic Acids Res., 9, 2433-2453.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.52 aurintricarboxylic acid
-
 Vigna unguiculata
2.7.7.52 Cibacron blue F3GA
-
 Vigna unguiculata
2.7.7.52 diphosphate
-
 Vigna unguiculata
2.7.7.52 heparin
-
 Vigna unguiculata
2.7.7.52 Ionic strength quite sensitive to ionic strength, activity decreases by 50% at about 40 mM (NH4)2SO4 or 125 mM potassium acetate Vigna unguiculata
2.7.7.52 additional information not inhibitory: actinomycin C, rifamycin, alpha-amanitin, phosphate Vigna unguiculata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.52 membrane
-
 Vigna unguiculata 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.52 Mg2+ divalent cation required, Mg2+ or Mn2+, 4-12 mM stimulate, optimal at 5 mM Vigna unguiculata
2.7.7.52 Mn2+ divalent cation required, Mg2+ or Mn2+, 1.1-1.5 mM stimulate Vigna unguiculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.52 50500
-
 velocity sedimentation Vigna unguiculata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.52 UTP + RNAn Vigna unguiculata RNA uridylyltransferase might function in uridylating specific proteins, RNA is not a natural substrate diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.52 Vigna unguiculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.52 614fold from uninfected and 117fold from CPMV-infected leaves Vigna unguiculata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.52 leaf
-
 Vigna unguiculata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.52 additional information
-
-
 Vigna unguiculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.52 UTP + RNAn the enzyme requires a single-stranded oligoribonucleotide or polyribonucleotide with a free terminal 3'-OH as primer, e.g. oligoA20, tRNAAsp, E. coli RNA, alfalafa mosaic virus RNA 4 Vigna unguiculata diphosphate + RNAn+1
-
 ?
2.7.7.52 UTP + RNAn marked specificity for UTP Vigna unguiculata diphosphate + RNAn+1
-
 ?
2.7.7.52 UTP + RNAn RNA substrate specificity, overview Vigna unguiculata diphosphate + RNAn+1
-
 ?
2.7.7.52 UTP + RNAn RNA uridylyltransferase might function in uridylating specific proteins, RNA is not a natural substrate Vigna unguiculata diphosphate + RNAn+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.52 30
-
-
 Vigna unguiculata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.52 30
-
 at least 3 h Vigna unguiculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.52 8.2
-
 assay at Vigna unguiculata