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Literature summary extracted from

  • Bruser, T.; Selmer, T.; Dahl, C.
    "ADP sulfurylase" from Thiobacillus denitrificans is an adenylylsulfate:phosphate adenylyltransferase and belongs to a new family of nucleotidyltransferases (2000), J. Biol. Chem., 275, 1691-1698.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.5
-
 Thiobacillus denitrificans

General Stability

EC Number General Stability Organism
2.7.7.5 purified enzyme stabilized by 5 mg/ml bovine serum albumin Thiobacillus denitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.5 ADP
-
 Thiobacillus denitrificans
2.7.7.5 diacetyl slow but complete inactivation Thiobacillus denitrificans
2.7.7.5 diethyldicarbonate
-
 Thiobacillus denitrificans
2.7.7.5 Phenylglyoxal incomplete inactivation Thiobacillus denitrificans
2.7.7.5 sulfate inhibitory at high concentrations Thiobacillus denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.5 0.3
-
 adenosine 5'-phosphosulfate pH 7.6 Thiobacillus denitrificans
2.7.7.5 12
-
 phosphate pH 7.6 Thiobacillus denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.5 Co2+ no effect Thiobacillus denitrificans
2.7.7.5 Cu2+ no effect Thiobacillus denitrificans
2.7.7.5 Fe2+ very slight inhibition Thiobacillus denitrificans
2.7.7.5 Mn2+ no effect Thiobacillus denitrificans
2.7.7.5 Zn2+ very slight inhibition Thiobacillus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.5 41000
-
 SDS-PAGE Thiobacillus denitrificans
2.7.7.5 41380
-
 MALDI-MS Thiobacillus denitrificans
2.7.7.5 41390
-
 calculated from DNA sequence Thiobacillus denitrificans
2.7.7.5 82000
-
 gel filtration Thiobacillus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.5 phosphate + adenosine 5'-phosphosulfate Thiobacillus denitrificans
-
 ADP + SO42-
-
 ?
2.7.7.5 phosphate + adenosine 5'-phosphosulfate Thiobacillus denitrificans RT
-
 ADP + SO42-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.5 Thiobacillus denitrificans Q9LA72
-
-
2.7.7.5 Thiobacillus denitrificans RT Q9LA72
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.5
-
 Thiobacillus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.5 2100
-
 purified enzyme, pH 7.6 Thiobacillus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.5 additional information no activity with ADP, CDP, GDP, UDP and IDP Thiobacillus denitrificans ?
-
 ?
2.7.7.5 additional information no activity with ADP, CDP, GDP, UDP and IDP Thiobacillus denitrificans RT ?
-
 ?
2.7.7.5 phosphate + adenosine 5'-phosphosulfate
-
 Thiobacillus denitrificans ADP + SO42-
-
 ?
2.7.7.5 phosphate + adenosine 5'-phosphosulfate ping pong reaction mechanism Thiobacillus denitrificans ADP + SO42-
-
 ir
2.7.7.5 phosphate + adenosine 5'-phosphosulfate highly specific for adenosine 5'-phosphosulfate Thiobacillus denitrificans ADP + SO42-
-
 ir
2.7.7.5 phosphate + adenosine 5'-phosphosulfate
-
 Thiobacillus denitrificans RT ADP + SO42-
-
 ?
2.7.7.5 phosphate + adenosine 5'-phosphosulfate ping pong reaction mechanism Thiobacillus denitrificans RT ADP + SO42-
-
 ir
2.7.7.5 phosphate + adenosine 5'-phosphosulfate highly specific for adenosine 5'-phosphosulfate Thiobacillus denitrificans RT ADP + SO42-
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.7.5 dimer 2 * 41000, gel filtration, SDS-PAGE Thiobacillus denitrificans

Synonyms

EC Number Synonyms Comment Organism
2.7.7.5 More the name sulfurylase should not be used because the reaction was found to be irreversible, enzyme catalyzes the formation of ADP and SO42- from phosphate and adenosine 5'-phosphosulfate Thiobacillus denitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.5 8.5 9
-
 Thiobacillus denitrificans