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Literature summary extracted from

  • Temin, H.M.; Mizutani, S.
    RNA-dependent DNA polymerase in virions of Rous sarcoma virus (1970), Nature, 226, 1211-1213.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.49 dithiothreitol to observe full activity of the enzyme, it is necessary to treat the virions with a non-ionic detergent. If the treatment is at 40°C the presence of dithiothreitol is necessary to recover activity Rous sarcoma virus
2.7.7.49 Nonionic detergent to observe full activity of the enzyme, it is necessary to treat the virions with a non-ionic detergent. If the treatment is at 40°C the presence of dithiothreitol is necessary to recover activity Rous sarcoma virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.49 KCl 80 mM, 60% inhibition Rous sarcoma virus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.49 K+ monovalent cation is not required for activity, 20 mM KCl causes 15% stimulation Rous sarcoma virus
2.7.7.49 Mg2+ absolute requirement, optimal concentration: 10 mM Rous sarcoma virus
2.7.7.49 Mn2+ MnCl2 can substitute for MgCl2 Rous sarcoma virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.49 Rous sarcoma virus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.49 deoxynucleoside triphosphate + DNAn all four deoxyribonucleotide triphosphates are required for full activity, some activity is present when only three deoxyribonucleotide triphosphates are added and 10-20% of full activity is still present with only two deoxyribonucleotide triphosphates Rous sarcoma virus diphosphate + DNAn+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.49 40 50
-
 Rous sarcoma virus