Literature summary extracted from
Xiao, M.; Wang, Y.; Chen, J.; Li, B.
Characterization of RNA-dependent RNA polymerase activity of CSFV NS5B proteins expressed in Escherichia coli (2003), Virus Genes, 27, 67-74.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.7.48 |
full-length enzyme and truncated enzyme forms resulting from deletions of 24, 36, 65 or 82 amino acid residues at the C terminal, with C-terminal hexahistidine tag, expression in Escherichia coli |
Classical swine fever virus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.7.48 |
additional information |
truncated enzyme forms resulting from deletions of 24, 36, or amino acid residues at the C terminal have RNA-dependent RNA polymerase activity. Truncated enzyme with a deletion of 82 amino acid residues at the C terminal shows no RNA-dependent RNA polymerase activity. Template specificity of the enzyme is not strict wirth NS5B proteins truncated, suggesting that the C terminal of CSFV NS5B protein is involved in the template specificity of the enzyme |
Classical swine fever virus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.48 |
Classical swine fever virus |
- |
expression in Escherichia coli |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.7.48 |
full-length enzyme and truncated enzyme forms resulting from deletions of 24, 36, 65 or 82 amino acid residues at the C terminal, with C-terminal hexahistidine tag |
Classical swine fever virus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.48 |
nucleoside triphosphate + RNAn |
- |
Classical swine fever virus |
diphosphate + RNAn+1 |
- |
? |
|