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Literature summary extracted from

  • Johnson, R.B.; Sun, X.L.; Hockman, M.A.; Villarreal, E.C.; Wakulchik, M.; Wang, Q.M.
    Specificity and mechanism analysis of Hepatitis C virus RNA-dependent RNA polymerase (2000), Arch. Biochem. Biophys., 377, 129-134.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 expressed as a nonfusion protein in Escherichia coli Hepacivirus C

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.48 additional information
-
 Hepacivirus C

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 Cu2+
-
 Hepacivirus C
2.7.7.48 heparin IC50: 80 ng/ml Hepacivirus C
2.7.7.48 NaCl 150 mM or above, about 90% of acctivity is lost Hepacivirus C
2.7.7.48 Ni2+
-
 Hepacivirus C
2.7.7.48 polylysine IC50: 250 ng/ml Hepacivirus C
2.7.7.48 Zn2+
-
 Hepacivirus C

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Ca2+ divalent cation required, Ca2+ shows about 20% of the activity with Mg2+ at about 1 mM Hepacivirus C
2.7.7.48 Mg2+ absolute requirement for divalent cation, effective concentration range is 5-10 mM Hepacivirus C
2.7.7.48 Mn2+ absolute requirement for divalent cation, effective concentration is about 1 mM Hepacivirus C
2.7.7.48 NaCl 15-40 mM, 2 to 4fold stimulation Hepacivirus C

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Hepacivirus C
-
 expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48
-
 Hepacivirus C

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
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