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Literature summary extracted from

  • Park, C.; Kee, Y.; Lee, J.; Oh, J.; Park, J.; Myung, H.
    Purification and characterization of recombinant Hepatitis C virus replicase (1999), J. Microbiol. Biotechnol., 9, 881-884.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 cloned and expressed in Escherichia coli with a C-terminal hexahistidine tag Hepacivirus C

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Hepacivirus C
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expressed in Escherichia coli
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 purified from Escherichia coli to near homogeneity. When the 21 amino acids from the C-terminus of the protein are deleted, an inclusion body is not formed and a better purification yield is achieved Hepacivirus C

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn ribonucleotide-incorporating activity on an in vitro transcribed RNA containing the 3' end of the HCV genome. It also possesses ribunucleotide incorporation activity, to a lesser extent, on in vitro transcribed foreign RNA templates when RNA or DNA primers are present. The activity is higher with DNA primers than with RNA primers Hepacivirus C diphosphate + RNAn+1
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