Literature summary extracted from
Park, C.; Kee, Y.; Lee, J.; Oh, J.; Park, J.; Myung, H.
Purification and characterization of recombinant Hepatitis C virus replicase (1999), J. Microbiol. Biotechnol., 9, 881-884.
No PubMed abstract available
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.7.48 |
cloned and expressed in Escherichia coli with a C-terminal hexahistidine tag |
Hepacivirus C |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.48 |
Hepacivirus C |
- |
expressed in Escherichia coli |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.7.48 |
purified from Escherichia coli to near homogeneity. When the 21 amino acids from the C-terminus of the protein are deleted, an inclusion body is not formed and a better purification yield is achieved |
Hepacivirus C |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.48 |
nucleoside triphosphate + RNAn |
ribonucleotide-incorporating activity on an in vitro transcribed RNA containing the 3' end of the HCV genome. It also possesses ribunucleotide incorporation activity, to a lesser extent, on in vitro transcribed foreign RNA templates when RNA or DNA primers are present. The activity is higher with DNA primers than with RNA primers |
Hepacivirus C |
diphosphate + RNAn+1 |
- |
? |
|