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Literature summary extracted from

  • Guarino, L.A.; Kaesberg, P.
    Isolation and characterization of an RNA-dependent RNA polymerase from Black beetle virus-infected Drosophila melanogaster cells (1981), J. Virol., 40, 379-386.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 Cl-
-
 black beetle virus
2.7.7.48 Mn2+ above 2 mM black beetle virus
2.7.7.48 RNase pancreatic RNAse and T1 RNAse black beetle virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.48 0.03
-
 UTP pH 8.2, 29°C black beetle virus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.48 additional information particulate material of cells of Drosophila melanogaster infected with Black beetle virus black beetle virus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 K+ slight stimulation by potassium acetate, optimal activity at 60 mM black beetle virus
2.7.7.48 Mg2+ required, optimal activity with 10-20 mM magnesium acetate black beetle virus
2.7.7.48 Mn2+ Mg2+ requirement can be partially replaced by Mn2+ black beetle virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 black beetle virus
-
 Drosophila melanogaster infected with
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48
-
 black beetle virus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.48 additional information cells of Drosophila melanogaster infected with Black beetle virus black beetle virus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.48 0.00000016
-
-
 black beetle virus

Storage Stability

EC Number Storage Stability Organism
2.7.7.48 -70°C, stable for several months black beetle virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn full-length negative strand BBV RNAs are synthesized black beetle virus diphosphate + RNAn+1
-
 ?
2.7.7.48 UTP + RNAn
-
 black beetle virus diphosphate + RNAn+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.48 29
-
-
 black beetle virus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.48 18 37 18°C: about 50% of maximal activity, 37°C: about 50% of maximal activity black beetle virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.48 8.2
-
-
 black beetle virus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.48 7.6 9 pH 7.6: about 70% of maximal activity, pH 9.0: about 80% of maximal activity black beetle virus