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Literature summary extracted from

  • Lohmann, V.; Roos, A.; Körner, F.; Koch, J.O.; Bartenschlager, R.
    Biochemical and kinetic analyses of NS5B RNA-dependent RNA polymerase of the Hepatitis C virus (1998), Virology, 249, 108-118.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 a hexahistidine affinity-tagged NS5B fusion protein is expressed with recombinant baculoviruses in insect cells Hepacivirus C

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.48 additional information a hexahistidine affinity-tagged NS5B fusion protein is expressed with recombinant baculoviruses in insect cells Hepacivirus C

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 NaCl at high concentrations Hepacivirus C
2.7.7.48 Zn2+ complete inhibition at concentrations above 1.25 mM ZnCl2 Hepacivirus C

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.48 0.00028
-
 CTP pH 7.5, 22°C, HCV 3'-end as template Hepacivirus C
2.7.7.48 0.00054
-
 GTP pH 7.5, 22°C, HCV 3'-end as template Hepacivirus C
2.7.7.48 0.0029
-
 GTP pH 7.5, 22°C, HCV 3'-end as template Hepacivirus C
2.7.7.48 0.00297
-
 GTP pH 7.5, 22°C, poly(C)/oligo(G) as template Hepacivirus C
2.7.7.48 0.0108
-
 ATP pH 7.5, 22°C, HCV 3'-end as template Hepacivirus C
2.7.7.48 0.022
-
 UTP pH 7.5, 22°C, poly(A)/oligo(U) as template Hepacivirus C

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 K+ optimal activity at 10 mM Hepacivirus C
2.7.7.48 Mg2+ absolutely dependent on, optimal activity at 12.5 mM MgCl2 Hepacivirus C
2.7.7.48 Mn2+ can replace for Mg2+, optimal concentration is 7-9 mM MnCl2 Hepacivirus C
2.7.7.48 NaCl optimal activity at 80-100 mM Hepacivirus C

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Hepacivirus C
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 a hexahistidine affinity-tagged NS5B fusion protein expressed with recombinant baculoviruses in insect cells Hepacivirus C

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 ATP + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
 ?
2.7.7.48 CTP + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
 ?
2.7.7.48 GTP + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.48 additional information
-
 additional information the enzyme is able to copy long heteropolymeric templates with an elongation rate of 150-200 nucleotides per min at 22°C Hepacivirus C

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.48 7
-
-
 Hepacivirus C