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Literature summary extracted from

  • Yonesaki, T.; Haruna, I.
    In vitro replication of Bacteriophage GA RNA. Subunit structure and catalytic properties of GA replicase (1981), J. Biochem., 89, 741-750.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 additional information activity is lowered at ionic strength of 0.1 Enterobacteria phage GA
2.7.7.48 RNase RNAse A, 0.01 mg/ml, or Rnase T1, 0.002 mg/ml, inhibit the reaction by 90-95% Enterobacteria phage GA

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Enterobacteria phage GA
-
 Escherichia coli infected with
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48
-
 Enterobacteria phage GA

Storage Stability

EC Number Storage Stability Organism
2.7.7.48 -20°C, 25% glycerol, half-life is 2 months Enterobacteria phage GA
2.7.7.48 0°C, 25% glycerol, half-life is 10 d Enterobacteria phage GA

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Enterobacteria phage GA diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.48 More the enzyme contains four different subunits: I, II, II and IV. The molecular weights of which are 74000 Da, 60000 Da, 47000 Da and 36000 Da, respectively. Three of them, I, III and IV are host-coded proteins, ribosomal protein S1 and elongation factor Tu(III) and Ts(IV) Enterobacteria phage GA