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Literature summary extracted from

  • Kumarasamy, R.; Symons, R.H.
    Extensive purification of the cucumber mosaic virus-induced RNA replicase (1979), Virology, 96, 622-632.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.48 soluble
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 Cucumber mosaic virus
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Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.48 additional information
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 a major polypeptide of 100000 Da is specific for the RNA replicase, determined by SDS-PAGE Cucumber mosaic virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Cucumber mosaic virus
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 Cucumis sativus seedlings infected with
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48
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 Cucumber mosaic virus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.48 0.012
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 activity with CMV RNA Cucumber mosaic virus
2.7.7.48 0.13
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 activity with poly(C) Cucumber mosaic virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn the enzyme copies CMV RNA and several other viral RNAs, Brome mosaic virus RNA, Alfalfa mosaic virus RNA and Tobacco mosaic virus RNA. Activity with poly(C) and poly(U) but not poly(A) or poly(G). The product with CMV RNA as template is heterogenous in size with a peak length of about 150 residues Cucumber mosaic virus diphosphate + RNAn+1
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