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Literature summary extracted from

  • Schiebel, W.; Haas, B.; Marinkovic, S.; Klanner, A.; Sänger, H.L.
    RNA-directed RNA polymerase from tomato leaves. I. Purification and physical properties (1993), J. Biol. Chem., 268, 11851-11857.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 heparin 0.0005 mM, 50% inhibition Solanum lycopersicum
2.7.7.48 KCl 100 mM, slight decrease in activity Solanum lycopersicum
2.7.7.48 NaCl slight decrease in activity Solanum lycopersicum
2.7.7.48 NEM 0.1 mM, reduces the transcription activity down to 10% Solanum lycopersicum
2.7.7.48 p-Hydroxymercuriphenylsulfonate 0.1 mM, reduces the transcription activity down to 10% Solanum lycopersicum
2.7.7.48 Sodium diphosphate 10 mM, decreases incorporation down to 5% Solanum lycopersicum
2.7.7.48 spermine 1 mM, 50% inhibition Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.48 additional information
-
 additional information the Km-value for the equimolar mixture of ATP, CTP and GTP in presence of 0.02 mM UTP is about 0.035 mM Solanum lycopersicum
2.7.7.48 0.004
-
 UTP in presence of 0.3 mM each of ATP, CTP and GTP Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 K+ KCl, activity is increased by a factor of about 1.2-1.5 when the final salt concentration is raised to 60 mM by the addition of NaCl, KCl or NH4Cl Solanum lycopersicum
2.7.7.48 Mg2+ maximal activity at 10 mM magnesium acetate Solanum lycopersicum
2.7.7.48 Mn2+ optimal activity at 1 mM, 20% of the activity obtained with Mg2+ Solanum lycopersicum
2.7.7.48 NaCl activity is increased by a factor of about 1.2-1.5 when the final salt concentration is raised to 60 mM by the addition of NaCl, KCl or NH4Cl Solanum lycopersicum
2.7.7.48 NH4Cl activity is increased by a factor of about 1.2-1.5 when the final salt concentration is raised to 60 mM by the addition of NaCl, KCl or NH4Cl Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.48 119000
-
 sucrose density gradient centrifugation Solanum lycopersicum
2.7.7.48 128000
-
 1 * 128000, SDS-PAGE Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Solanum lycopersicum
-
 inoculated with potato spindle tuber viroid. In viroid-infected tomato leaf the activity of the host-encoded RdRP is significantly increased. Viroids are not translated into proteins so that they cannot code for a viroid-specific RNA replicase
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.48 leaf leaf inoculated with potato spindle tuber viroid. In viroid-infected tomato leaf the activity of the host-encoded RdRP is significantly increased. Viroids are not translated into proteins so that they cannot code for a viroid-specific RNA replicase Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.48 0.0166
-
-
 Solanum lycopersicum

Storage Stability

EC Number Storage Stability Organism
2.7.7.48 -20°C, 50% glycerol-containing buffer, activity decreases by about 15% per month Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Solanum lycopersicum diphosphate + RNAn+1
-
 ?
2.7.7.48 UTP + RNAn
-
 Solanum lycopersicum diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.48 monomer 1 * 128000, SDS-PAGE Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.48 37
-
-
 Solanum lycopersicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.48 27 37 27°C: about 50% of maximal activity, 37°C: optimum Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.48 7.8
-
-
 Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.48 7 9 pH 7.0: about 70% of maximal activity, pH 9.0: about 40% of maximal activity Solanum lycopersicum