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Literature summary extracted from

  • Traub, A.; Diskin, B.; Rosenberg, H.; Kalmar, E.
    Isolation and properties of the replicase of Encephalomyocarditis virus (1976), J. Virol., 18, 375-382.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.7.48 the isolated enzyme is very labile Encephalomyocarditis virus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.48 additional information the enzyme is closely associated with the smooth membranes of infected BHK-21 cells Encephalomyocarditis virus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mg2+ strict dependence on Mg2+ Encephalomyocarditis virus
2.7.7.48 Mn2+ can not efficiently replace Mg2+ Encephalomyocarditis virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Encephalomyocarditis virus
-
 BHK-21 cells infected with
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48
-
 Encephalomyocarditis virus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.48 0.00184
-
-
 Encephalomyocarditis virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn the enzyme does not manifest strict specificity towards EMC RNA template. It can use also Qbeta RNA, rRNA of BHK cells or poly(C) Encephalomyocarditis virus diphosphate + RNAn+1
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.48 8
-
-
 Encephalomyocarditis virus