Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Blumenthal, T.
    Qbeta replicase (1982), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 15, 267-279.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.48 host factor hexameric host-coded protein of 12500 Da subunits is required for in vitro Qbeta RNA replication Qubevirus durum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 aurintricarboxylic acid inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains Qubevirus durum
2.7.7.48 GTP competitive inhibitor of initiation but not of elongation Qubevirus durum
2.7.7.48 Poly(U) inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains Qubevirus durum
2.7.7.48 Polyethylene sulfonate inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains Qubevirus durum
2.7.7.48 ppGpp competitive inhibitor of initiation but not of elongation Qubevirus durum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mn2+ Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template Qubevirus durum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.48 35000
-
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts Qubevirus durum
2.7.7.48 130000
-
the low value compared to the sum of molecular weights of the four subunits is probably a result of an oblate shape conferred by ribosomal protein S1, glycerol gradient sedimentation Qubevirus durum
2.7.7.48 215000
-
SDS-PAGE of four polypeptides covalently cross-linked Qubevirus durum
2.7.7.48 450000
-
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts Qubevirus durum
2.7.7.48 650000
-
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts Qubevirus durum
2.7.7.48 700000
-
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts Qubevirus durum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Qubevirus durum
-
Escherichia coli infected with
-
2.7.7.48 Qubevirus durum
-
QbetaamB86
-
2.7.7.48 Qubevirus durum
-
Qbetaam12
-
2.7.7.48 Qubevirus durum Qbetaam12
-
Qbetaam12
-
2.7.7.48 Qubevirus durum QbetaamB86
-
QbetaamB86
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48
-
Qubevirus durum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template Qubevirus durum diphosphate + RNAn+1
-
?
2.7.7.48 nucleoside triphosphate + RNAn GTP and polyC Qubevirus durum diphosphate + RNAn+1
-
?
2.7.7.48 nucleoside triphosphate + RNAn synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template Qubevirus durum Qbetaam12 diphosphate + RNAn+1
-
?
2.7.7.48 nucleoside triphosphate + RNAn GTP and polyC Qubevirus durum Qbetaam12 diphosphate + RNAn+1
-
?
2.7.7.48 nucleoside triphosphate + RNAn synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template Qubevirus durum QbetaamB86 diphosphate + RNAn+1
-
?
2.7.7.48 nucleoside triphosphate + RNAn GTP and polyC Qubevirus durum QbetaamB86 diphosphate + RNAn+1
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.48 tetramer x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts Qubevirus durum