EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.48 | host factor | hexameric host-coded protein of 12500 Da subunits is required for in vitro Qbeta RNA replication | Qubevirus durum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.48 | aurintricarboxylic acid | inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains | Qubevirus durum | |
2.7.7.48 | GTP | competitive inhibitor of initiation but not of elongation | Qubevirus durum | |
2.7.7.48 | Poly(U) | inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains | Qubevirus durum | |
2.7.7.48 | Polyethylene sulfonate | inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains | Qubevirus durum | |
2.7.7.48 | ppGpp | competitive inhibitor of initiation but not of elongation | Qubevirus durum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.48 | Mn2+ | Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template | Qubevirus durum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.48 | 35000 | - |
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts | Qubevirus durum |
2.7.7.48 | 130000 | - |
the low value compared to the sum of molecular weights of the four subunits is probably a result of an oblate shape conferred by ribosomal protein S1, glycerol gradient sedimentation | Qubevirus durum |
2.7.7.48 | 215000 | - |
SDS-PAGE of four polypeptides covalently cross-linked | Qubevirus durum |
2.7.7.48 | 450000 | - |
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts | Qubevirus durum |
2.7.7.48 | 650000 | - |
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts | Qubevirus durum |
2.7.7.48 | 700000 | - |
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts | Qubevirus durum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.48 | Qubevirus durum | - |
Escherichia coli infected with | - |
2.7.7.48 | Qubevirus durum | - |
QbetaamB86 | - |
2.7.7.48 | Qubevirus durum | - |
Qbetaam12 | - |
2.7.7.48 | Qubevirus durum Qbetaam12 | - |
Qbetaam12 | - |
2.7.7.48 | Qubevirus durum QbetaamB86 | - |
QbetaamB86 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.48 | - |
Qubevirus durum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.48 | nucleoside triphosphate + RNAn | synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template | Qubevirus durum | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | nucleoside triphosphate + RNAn | GTP and polyC | Qubevirus durum | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | nucleoside triphosphate + RNAn | synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template | Qubevirus durum Qbetaam12 | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | nucleoside triphosphate + RNAn | GTP and polyC | Qubevirus durum Qbetaam12 | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | nucleoside triphosphate + RNAn | synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template | Qubevirus durum QbetaamB86 | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | nucleoside triphosphate + RNAn | GTP and polyC | Qubevirus durum QbetaamB86 | diphosphate + RNAn+1 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.48 | tetramer | x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts | Qubevirus durum |