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Literature summary extracted from

  • Cartwright, J.L.; McLennan, A.G.
    GTP:GTP guanylyl transferase: trapping procedures for detecting and characterizing chemical nature of enzyme-nucleotide phosphoramidate reaction intermediate (2002), Methods Enzymol., 354, 251-260.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.45 110000
-
 x * 110000, SDS-PAGE Artemia franciscana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.45 GTP + GTP Artemia franciscana
-
 diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate
-
 r
2.7.7.45 GTP + GTP Daphnia magna
-
 diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.45 Artemia franciscana
-
-
-
2.7.7.45 Daphnia magna
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.45
-
 Artemia franciscana

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.45 2 GTP = diphosphate + P1,P4-bis(5'-guanosyl) tetraphosphate the alpha-phosphorus group of the GMP of the covalent reaction intermediate is linked via the Nepsilon2 ring nitrogen of a histidine residue of the enzyme Artemia franciscana
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.45 cyst
-
 Artemia franciscana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.45 GTP + GTP
-
 Daphnia magna P1,P4-bis(5'-guanosyl)tetraphosphate + diphosphate i.e. GP4G r
2.7.7.45 GTP + GTP the alpha-phosphorus group of the GMP of the covalent reaction intermediate is linked to the Nepsilon2 ring nitrogen of a histidine residue of the enzyme Artemia franciscana P1,P4-bis(5'-guanosyl)tetraphosphate + diphosphate i.e. GP4G r
2.7.7.45 GTP + GTP
-
 Artemia franciscana diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate
-
 r
2.7.7.45 GTP + GTP
-
 Daphnia magna diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate
-
 r
2.7.7.45 GTP + ITP
-
 Artemia franciscana GppppI + diphosphate
-
 r
2.7.7.45 GTP + XTP
-
 Artemia franciscana GppppX + diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.45 ? x * 110000, SDS-PAGE Artemia franciscana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.45 20
-
 assay at Artemia franciscana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.45 37
-
 20 min, 4 M sodium acetate, pH 7.5, 96% of maximal activity Artemia franciscana
2.7.7.45 37
-
 20 min, 3.68 M hydroxylamine, pH 4.75, 0.7% of maximal activity Artemia franciscana
2.7.7.45 37
-
 20 min, 0.2 M hydroxylamine, pH 7.5, 2.5% of maximal activity Artemia franciscana
2.7.7.45 85
-
 5 min: stable at alkaline conditions of 0.15 M NaOH, 79.1% of maximal activity at pH 7.5, 1% of maximal activity at acidic conditions of 0.15 M HCl Artemia franciscana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.45 additional information
-
 stable at alkaline pH conditions at 85% for 5 min Artemia franciscana
2.7.7.45 additional information
-
 unstable at acidic pH conditions Artemia franciscana
2.7.7.45 additional information
-
 stability of the enzyme-guanylate reaction intermediate complex at different temperatures and pH values Artemia franciscana