BRENDA - Enzyme Database

Extraction and stabilization of mammalian CDP-diacylglycerol synthase activity

Monaco, M.E.; Feldman, M.; Biochem. Biophys. Res. Commun. 239, 166-170 (1997)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
bovine serum albumin
activity is 6fold increased
Rattus norvegicus
2.7.7.41
GTP
activity is 2fold enhanced
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
CDP-diacylglycerol
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
additional information
-
additional information
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
required
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.41
liver
-
Rattus norvegicus
-
Storage Stability
EC Number
Storage Stability
Organism
2.7.7.41
-72°C, the enzyme extract from microsomal membranes is indefinitely stable
Rattus norvegicus
2.7.7.41
4°C, the enzyme extract from microsomal membranes is stable for at least 24 h
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
-
643322
Rattus norvegicus
diphosphate + CDPdiacylglycerol
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.7.41
22
-
24 h, enzyme in extract from microsomal membranes loses 50% of its activity
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
bovine serum albumin
activity is 6fold increased
Rattus norvegicus
2.7.7.41
GTP
activity is 2fold enhanced
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
CDP-diacylglycerol
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
additional information
-
additional information
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
required
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.41
liver
-
Rattus norvegicus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.7.41
-72°C, the enzyme extract from microsomal membranes is indefinitely stable
Rattus norvegicus
2.7.7.41
4°C, the enzyme extract from microsomal membranes is stable for at least 24 h
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
-
643322
Rattus norvegicus
diphosphate + CDPdiacylglycerol
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.7.41
22
-
24 h, enzyme in extract from microsomal membranes loses 50% of its activity
Rattus norvegicus