BRENDA - Enzyme Database

Purification and characterization of CDP-diacylglycerol synthase from Saccharomyces cerevisiae

Kelley, M.J.; Carman, G.M.; J. Biol. Chem. 262, 14563-14570 (1987)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
Triton X-100
maximal stimulation at 15 mM
Saccharomyces cerevisiae
General Stability
EC Number
General Stability
Organism
2.7.7.41
stable to at least 2 cycles of freezing and thawing
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
dCTP
competitive with respect to CTP
Saccharomyces cerevisiae
2.7.7.41
Hg2+
5 mM HgCl2, 78% inhibition
Saccharomyces cerevisiae
2.7.7.41
PCMB
5 mM, 88% inhibition
Saccharomyces cerevisiae
2.7.7.41
Triton X-100
above 20 mM
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.5
-
phosphatidate
pH 6.5, 30°C
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
mitochondrial membrane
-
Saccharomyces cerevisiae
31966
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
maximal activity at 20 mM Mg2+
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.41
114000
-
radiation inactivation
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.41
-
Saccharomyces cerevisiae
Reaction
EC Number
Reaction
Commentary
Organism
2.7.7.41
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
sequential bi bi reaction mechanism
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
1.409
-
-
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
2.7.7.41
-80°C, in presence of 1 mM CTP the purified enzyme is 90-100% stable for at least 3 months
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
equilibrium constant is 0.001. Reverse reaction is favored in vitro
643309
Saccharomyces cerevisiae
diphosphate + CDPdiacylglycerol
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
2.7.7.41
More
two polypeptides of 54000 Da and 56000 Da are detected by SDS-PAGE, it is possible that the 54000 Da subunit is a proteolysis product of the 56000 Da subunit
Saccharomyces cerevisiae
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.7.41
30
-
20 min, labile above
Saccharomyces cerevisiae
2.7.7.41
60
-
20 min, complete inactivation
Saccharomyces cerevisiae
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.7.41
8.92
-
CTP
pH 6.5, 30°C
Saccharomyces cerevisiae
2.7.7.41
8.92
-
phosphatidate
pH 6.5, 30°C
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
6.5
-
-
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.41
6
8.5
pH 6.0: 70% of maximal activity, pH 8.5: about 70% of maximal activity
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
Triton X-100
maximal stimulation at 15 mM
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.41
stable to at least 2 cycles of freezing and thawing
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
dCTP
competitive with respect to CTP
Saccharomyces cerevisiae
2.7.7.41
Hg2+
5 mM HgCl2, 78% inhibition
Saccharomyces cerevisiae
2.7.7.41
PCMB
5 mM, 88% inhibition
Saccharomyces cerevisiae
2.7.7.41
Triton X-100
above 20 mM
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.5
-
phosphatidate
pH 6.5, 30°C
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
mitochondrial membrane
-
Saccharomyces cerevisiae
31966
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
maximal activity at 20 mM Mg2+
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.41
114000
-
radiation inactivation
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.41
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
1.409
-
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.7.41
-80°C, in presence of 1 mM CTP the purified enzyme is 90-100% stable for at least 3 months
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
equilibrium constant is 0.001. Reverse reaction is favored in vitro
643309
Saccharomyces cerevisiae
diphosphate + CDPdiacylglycerol
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.7.41
More
two polypeptides of 54000 Da and 56000 Da are detected by SDS-PAGE, it is possible that the 54000 Da subunit is a proteolysis product of the 56000 Da subunit
Saccharomyces cerevisiae
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.7.41
30
-
20 min, labile above
Saccharomyces cerevisiae
2.7.7.41
60
-
20 min, complete inactivation
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.7.41
8.92
-
CTP
pH 6.5, 30°C
Saccharomyces cerevisiae
2.7.7.41
8.92
-
phosphatidate
pH 6.5, 30°C
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
6.5
-
-
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.41
6
8.5
pH 6.0: 70% of maximal activity, pH 8.5: about 70% of maximal activity
Saccharomyces cerevisiae