BRENDA - Enzyme Database

Characterization of cytidine triphosphate:phosphatidate cytidyltransferase from suspension cultured Catharanthus roseus cells

Hanenberg, A.; Heim, S.; Wissing, J.B.; Wagner, K.G.; Plant Sci. 88, 13-18 (1993)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
ATP
slight stimulation at high concentrations
Catharanthus roseus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
ATP
slight inhibition below 0.175 mM
Catharanthus roseus
2.7.7.41
CDP
-
Catharanthus roseus
2.7.7.41
diphosphate
-
Catharanthus roseus
2.7.7.41
additional information
no inhibition by 1.65 mM ADP
Catharanthus roseus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.08
-
phosphatidic acid
pH 7.2, 37°C
Catharanthus roseus
2.7.7.41
0.17
-
CTP
pH 7.2, 37°C
Catharanthus roseus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
chloroplast envelope
-
Catharanthus roseus
9941
-
2.7.7.41
endoplasmic reticulum
-
Catharanthus roseus
5783
-
2.7.7.41
microsome
-
Catharanthus roseus
-
-
2.7.7.41
plasma membrane
-
Catharanthus roseus
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Ca2+
activates at low concentrations, decrease in activity at higher concentrations
Catharanthus roseus
2.7.7.41
Co2+
activates at low concentrations, decrease in activity at higher concentrations
Catharanthus roseus
2.7.7.41
Mg2+
maximal activation, about 15fold, at 10 mM
Catharanthus roseus
2.7.7.41
Mn2+
maximal activation, about 10fold, at 1 mM
Catharanthus roseus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Catharanthus roseus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.41
-
Catharanthus roseus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.41
cell suspension culture
-
Catharanthus roseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
-
643307
Catharanthus roseus
diphosphate + CDPdiacylglycerol
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
7.3
-
-
Catharanthus roseus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.41
0.24
-
CDP
pH 7.2, 37°C
Catharanthus roseus
2.7.7.41
1.5
-
diphosphate
pH 7.2, 37°C
Catharanthus roseus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
ATP
slight stimulation at high concentrations
Catharanthus roseus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
ATP
slight inhibition below 0.175 mM
Catharanthus roseus
2.7.7.41
CDP
-
Catharanthus roseus
2.7.7.41
diphosphate
-
Catharanthus roseus
2.7.7.41
additional information
no inhibition by 1.65 mM ADP
Catharanthus roseus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.41
0.24
-
CDP
pH 7.2, 37°C
Catharanthus roseus
2.7.7.41
1.5
-
diphosphate
pH 7.2, 37°C
Catharanthus roseus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.08
-
phosphatidic acid
pH 7.2, 37°C
Catharanthus roseus
2.7.7.41
0.17
-
CTP
pH 7.2, 37°C
Catharanthus roseus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
chloroplast envelope
-
Catharanthus roseus
9941
-
2.7.7.41
endoplasmic reticulum
-
Catharanthus roseus
5783
-
2.7.7.41
microsome
-
Catharanthus roseus
-
-
2.7.7.41
plasma membrane
-
Catharanthus roseus
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Ca2+
activates at low concentrations, decrease in activity at higher concentrations
Catharanthus roseus
2.7.7.41
Co2+
activates at low concentrations, decrease in activity at higher concentrations
Catharanthus roseus
2.7.7.41
Mg2+
maximal activation, about 15fold, at 10 mM
Catharanthus roseus
2.7.7.41
Mn2+
maximal activation, about 10fold, at 1 mM
Catharanthus roseus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.41
-
Catharanthus roseus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.41
cell suspension culture
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
-
643307
Catharanthus roseus
diphosphate + CDPdiacylglycerol
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
7.3
-
-
Catharanthus roseus