BRENDA - Enzyme Database

Specific inhibition of phosphatidate cytidylyltransferase from Bacillus subtilis membranes by cytidine monophosphate

Gaillard, J.L.; Lubochinsky, B.; Rigomier, D.; Biochim. Biophys. Acta 753, 372-380 (1983)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
non-ionic detergent
absolute requirement
Bacillus subtilis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
CMP
-
Bacillus subtilis
2.7.7.41
diphosphate
-
Bacillus subtilis
2.7.7.41
Hg2+
0.1 mM, 90% inhibition
Bacillus subtilis
2.7.7.41
Mg2+
33% inhibition at 25 mM Mg2+
Bacillus subtilis
2.7.7.41
PCMB
0.03 mM, 90% inhibition
Bacillus subtilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.26
-
CTP
pH 7.5, 25°C
Bacillus subtilis
2.7.7.41
0.26
-
dCTP
pH 7.5, 25°C
Bacillus subtilis
2.7.7.41
0.3
-
phosphatidic acid
pH 7.5, 25°C, with 0.45 mM CTP
Bacillus subtilis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
membrane
localized exclusively in the membrane fraction
Bacillus subtilis
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
K+
stimulating effect increases with concentrations up to 75 mM
Bacillus subtilis
2.7.7.41
Mg2+
maximal activity with 3-6 mM. 33% inhibition at 25 mM
Bacillus subtilis
2.7.7.41
Mn2+
1 mM, 70% of the maximal activity obtained with Mg2+
Bacillus subtilis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Bacillus subtilis
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
2.7.7.41
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
ping-pong mechanism
Bacillus subtilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + 1,2-dipalmitoyl phosphatidic acid
-
643306
Bacillus subtilis
diphosphate + CDP-dipalmitoylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
-
643306
Bacillus subtilis
diphosphate + CDPdiacylglycerol
-
-
-
?
2.7.7.41
dCTP + phosphatidate
-
643306
Bacillus subtilis
diphosphate + dCDPdiacylglycerol
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.7.41
40
-
with detergent already inactivated at
Bacillus subtilis
2.7.7.41
60
-
30 min, membranous enzyme, stable up to
Bacillus subtilis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
7.5
-
-
Bacillus subtilis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.41
6.2
8.7
pH 6.2: 54% of maximal activity, pH 8.7: 56% of maximal activity
Bacillus subtilis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.41
0.75
-
diphosphate
pH 7.5, 25°C
Bacillus subtilis
2.7.7.41
5.6
-
CMP
pH 7.5, 25°C
Bacillus subtilis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
non-ionic detergent
absolute requirement
Bacillus subtilis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
CMP
-
Bacillus subtilis
2.7.7.41
diphosphate
-
Bacillus subtilis
2.7.7.41
Hg2+
0.1 mM, 90% inhibition
Bacillus subtilis
2.7.7.41
Mg2+
33% inhibition at 25 mM Mg2+
Bacillus subtilis
2.7.7.41
PCMB
0.03 mM, 90% inhibition
Bacillus subtilis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.41
0.75
-
diphosphate
pH 7.5, 25°C
Bacillus subtilis
2.7.7.41
5.6
-
CMP
pH 7.5, 25°C
Bacillus subtilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.26
-
CTP
pH 7.5, 25°C
Bacillus subtilis
2.7.7.41
0.26
-
dCTP
pH 7.5, 25°C
Bacillus subtilis
2.7.7.41
0.3
-
phosphatidic acid
pH 7.5, 25°C, with 0.45 mM CTP
Bacillus subtilis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
membrane
localized exclusively in the membrane fraction
Bacillus subtilis
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
K+
stimulating effect increases with concentrations up to 75 mM
Bacillus subtilis
2.7.7.41
Mg2+
maximal activity with 3-6 mM. 33% inhibition at 25 mM
Bacillus subtilis
2.7.7.41
Mn2+
1 mM, 70% of the maximal activity obtained with Mg2+
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + 1,2-dipalmitoyl phosphatidic acid
-
643306
Bacillus subtilis
diphosphate + CDP-dipalmitoylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
-
643306
Bacillus subtilis
diphosphate + CDPdiacylglycerol
-
-
-
?
2.7.7.41
dCTP + phosphatidate
-
643306
Bacillus subtilis
diphosphate + dCDPdiacylglycerol
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.7.41
40
-
with detergent already inactivated at
Bacillus subtilis
2.7.7.41
60
-
30 min, membranous enzyme, stable up to
Bacillus subtilis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
7.5
-
-
Bacillus subtilis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.41
6.2
8.7
pH 6.2: 54% of maximal activity, pH 8.7: 56% of maximal activity
Bacillus subtilis