BRENDA - Enzyme Database

CDP-diacylglycerol synthase activity in Clostridium perfringens

Carman, G.M.; Zaniewski, R.L.; Cousminer, J.J.; Appl. Environ. Microbiol. 43, 81-85 (1982)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
Triton X-100
5 mM, about 9fold stimulation
Clostridium perfringens
General Stability
EC Number
General Stability
Organism
2.7.7.41
stable to at least 2 cycles of freezing and thawing
Clostridium perfringens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.18
-
CTP
pH 7.0, 30C
Clostridium perfringens
2.7.7.41
0.22
-
phosphatidic acid
pH 7.0, 30C
Clostridium perfringens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
cell envelope
associated with cell envelope fraction
Clostridium perfringens
30313
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
divalent cation required, Mn2+ or Mg2+, maximal activity at 5 mM Mg2+. Maximal activity obtained with Mg2+ is about 1.2fold greater than that obtained with Mn2+
Clostridium perfringens
2.7.7.41
Mn2+
divalent cation required, Mn2+ or Mg2+, maximal activity at 1 mM Mn2+
Clostridium perfringens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Clostridium perfringens
-
-
-
Storage Stability
EC Number
Storage Stability
Organism
2.7.7.41
-80C, cell envelope preparations stable for at least 2 months
Clostridium perfringens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
-
643303
Clostridium perfringens
diphosphate + CDPdiacylglycerol
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.7.41
50
-
-
Clostridium perfringens
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
2.7.7.41
20
60
20C: about 35% of maximal activity, 60C: about 45% of maximal activity
Clostridium perfringens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
6.5
7.5
-
Clostridium perfringens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
Triton X-100
5 mM, about 9fold stimulation
Clostridium perfringens
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.41
stable to at least 2 cycles of freezing and thawing
Clostridium perfringens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.18
-
CTP
pH 7.0, 30C
Clostridium perfringens
2.7.7.41
0.22
-
phosphatidic acid
pH 7.0, 30C
Clostridium perfringens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
cell envelope
associated with cell envelope fraction
Clostridium perfringens
30313
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
divalent cation required, Mn2+ or Mg2+, maximal activity at 5 mM Mg2+. Maximal activity obtained with Mg2+ is about 1.2fold greater than that obtained with Mn2+
Clostridium perfringens
2.7.7.41
Mn2+
divalent cation required, Mn2+ or Mg2+, maximal activity at 1 mM Mn2+
Clostridium perfringens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.7.41
-80C, cell envelope preparations stable for at least 2 months
Clostridium perfringens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
-
643303
Clostridium perfringens
diphosphate + CDPdiacylglycerol
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.7.41
50
-
-
Clostridium perfringens
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
2.7.7.41
20
60
20C: about 35% of maximal activity, 60C: about 45% of maximal activity
Clostridium perfringens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
6.5
7.5
-
Clostridium perfringens