Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Carman, G.M.; Zaniewski, R.L.; Cousminer, J.J.
    CDP-diacylglycerol synthase activity in Clostridium perfringens (1982), Appl. Environ. Microbiol., 43, 81-85.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.41 Triton X-100 5 mM, about 9fold stimulation Clostridium perfringens

General Stability

EC Number General Stability Organism
2.7.7.41 stable to at least 2 cycles of freezing and thawing Clostridium perfringens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.41 0.18
-
 CTP pH 7.0, 30°C Clostridium perfringens
2.7.7.41 0.22
-
 phosphatidic acid pH 7.0, 30°C Clostridium perfringens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.41 cell envelope associated with cell envelope fraction Clostridium perfringens 30313
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.41 Mg2+ divalent cation required, Mn2+ or Mg2+, maximal activity at 5 mM Mg2+. Maximal activity obtained with Mg2+ is about 1.2fold greater than that obtained with Mn2+ Clostridium perfringens
2.7.7.41 Mn2+ divalent cation required, Mn2+ or Mg2+, maximal activity at 1 mM Mn2+ Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.41 Clostridium perfringens
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.7.7.41 -80°C, cell envelope preparations stable for at least 2 months Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.41 CTP + phosphatidate
-
 Clostridium perfringens diphosphate + CDPdiacylglycerol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.41 50
-
-
 Clostridium perfringens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.41 20 60 20°C: about 35% of maximal activity, 60°C: about 45% of maximal activity Clostridium perfringens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.41 6.5 7.5
-
 Clostridium perfringens