BRENDA - Enzyme Database

CDPdiacylglycerol synthase from yeast

Carman, G.M.; Kelley, M.J.; Methods Enzymol. 209, 242-247 (1992)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.7.41
stable to at least 2 cycles of freezing and thawing
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
dCTP
competitive with respect to CTP
Saccharomyces cerevisiae
2.7.7.41
Thiophosphatidate
competitive
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.5
-
phosphatidate
-
Saccharomyces cerevisiae
2.7.7.41
1
-
CTP
pH 6.5, 30°C
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
microsome
about 20% of enzyme is associated with
Saccharomyces cerevisiae
-
-
2.7.7.41
mitochondrion
main site of localization
Saccharomyces cerevisiae
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.41
114000
-
-
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.41
mitochondrial enzyme
Saccharomyces cerevisiae
Reaction
EC Number
Reaction
Commentary
Organism
2.7.7.41
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
sequential bi-bi reaction: CDPdiacylglycerol synthase binds to CTP prior to phosphatidate and diphosphate is released prior to CDPdiacylglycerol in the reaction sequence
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
additional information
-
-
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
2.7.7.41
-80°C, in presence of 1 mM CTP the purified enzyme is 90-100% stable for at least 3 months
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
-
643301
Saccharomyces cerevisiae
diphosphate + CDPdiacylglycerol
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.7.41
30
-
20 min, labile above
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.41
stable to at least 2 cycles of freezing and thawing
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
dCTP
competitive with respect to CTP
Saccharomyces cerevisiae
2.7.7.41
Thiophosphatidate
competitive
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.5
-
phosphatidate
-
Saccharomyces cerevisiae
2.7.7.41
1
-
CTP
pH 6.5, 30°C
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
microsome
about 20% of enzyme is associated with
Saccharomyces cerevisiae
-
-
2.7.7.41
mitochondrion
main site of localization
Saccharomyces cerevisiae
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.41
114000
-
-
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.41
mitochondrial enzyme
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
additional information
-
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.7.41
-80°C, in presence of 1 mM CTP the purified enzyme is 90-100% stable for at least 3 months
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
-
643301
Saccharomyces cerevisiae
diphosphate + CDPdiacylglycerol
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.7.41
30
-
20 min, labile above
Saccharomyces cerevisiae