BRENDA - Enzyme Database

Purification of CDPdiacylglycerol synthase from Escherichia coli

Sparrow, C.P.; Methods Enzymol. 209, 237-242 (1992)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.7.41
the pure enzyme can be dialyzed, but to maintain activity the dialyzing buffer must contain 1% n-octyl-beta-D-glucopyranoside, 0.25 mM dithiothreitol and at least 200 mM salt
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.28
-
phosphatidic acid
-
Escherichia coli
2.7.7.41
0.58
-
dCTP
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.41
27000
-
x * 27000, SDS-PAGE
Escherichia coli
2.7.7.41
27570
-
x * 27570, calculation from gene sequence
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.41
CTP + phosphatidate
Escherichia coli
the activity is essential for all phospholipid biosynthesis in Escherichia coli
diphosphate + CDPdiacylglycerol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.41
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
30.79
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
2.7.7.41
purified enzyme can be stored frozen for months if quick-frozen in liquid nitrogen
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
the enzyme is specific for long-chain phosphatidic acid. 1-Acyl-sn-glycero-3-phosphate is not a substrate and phosphatidic acids with acyl chains shorter than 16 carbons are poor substrates
643300
Escherichia coli
diphosphate + CDPdiacylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
the activity is essential for all phospholipid biosynthesis in Escherichia coli
643300
Escherichia coli
diphosphate + CDPdiacylglycerol
-
-
-
?
2.7.7.41
dCTP + phosphatidate
-
643300
Escherichia coli
diphosphate + dCDPdiacylglycerol
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.7.41
?
x * 27000, SDS-PAGE; x * 27570, calculation from gene sequence
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
7
8
-
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.41
the pure enzyme can be dialyzed, but to maintain activity the dialyzing buffer must contain 1% n-octyl-beta-D-glucopyranoside, 0.25 mM dithiothreitol and at least 200 mM salt
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.28
-
phosphatidic acid
-
Escherichia coli
2.7.7.41
0.58
-
dCTP
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.41
27000
-
x * 27000, SDS-PAGE
Escherichia coli
2.7.7.41
27570
-
x * 27570, calculation from gene sequence
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.41
CTP + phosphatidate
Escherichia coli
the activity is essential for all phospholipid biosynthesis in Escherichia coli
diphosphate + CDPdiacylglycerol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.41
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
30.79
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.7.41
purified enzyme can be stored frozen for months if quick-frozen in liquid nitrogen
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
the enzyme is specific for long-chain phosphatidic acid. 1-Acyl-sn-glycero-3-phosphate is not a substrate and phosphatidic acids with acyl chains shorter than 16 carbons are poor substrates
643300
Escherichia coli
diphosphate + CDPdiacylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
the activity is essential for all phospholipid biosynthesis in Escherichia coli
643300
Escherichia coli
diphosphate + CDPdiacylglycerol
-
-
-
?
2.7.7.41
dCTP + phosphatidate
-
643300
Escherichia coli
diphosphate + dCDPdiacylglycerol
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.7.41
?
x * 27000, SDS-PAGE; x * 27570, calculation from gene sequence
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
7
8
-
Escherichia coli