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Literature summary extracted from

  • Sparrow, C.P.
    Purification of CDPdiacylglycerol synthase from Escherichia coli (1992), Methods Enzymol., 209, 237-242.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.41 the pure enzyme can be dialyzed, but to maintain activity the dialyzing buffer must contain 1% n-octyl-beta-D-glucopyranoside, 0.25 mM dithiothreitol and at least 200 mM salt Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.41 0.28
-
phosphatidic acid
-
Escherichia coli
2.7.7.41 0.58
-
dCTP
-
Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.41 27000
-
x * 27000, SDS-PAGE Escherichia coli
2.7.7.41 27570
-
x * 27570, calculation from gene sequence Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.41 CTP + phosphatidate Escherichia coli the activity is essential for all phospholipid biosynthesis in Escherichia coli diphosphate + CDPdiacylglycerol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.41 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.41
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.41 30.79
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.41 purified enzyme can be stored frozen for months if quick-frozen in liquid nitrogen Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.41 CTP + phosphatidate the enzyme is specific for long-chain phosphatidic acid. 1-Acyl-sn-glycero-3-phosphate is not a substrate and phosphatidic acids with acyl chains shorter than 16 carbons are poor substrates Escherichia coli diphosphate + CDPdiacylglycerol
-
?
2.7.7.41 CTP + phosphatidate the activity is essential for all phospholipid biosynthesis in Escherichia coli Escherichia coli diphosphate + CDPdiacylglycerol
-
?
2.7.7.41 dCTP + phosphatidate
-
Escherichia coli diphosphate + dCDPdiacylglycerol
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.41 ? x * 27000, SDS-PAGE Escherichia coli
2.7.7.41 ? x * 27570, calculation from gene sequence Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.41 7 8
-
Escherichia coli