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Literature summary extracted from

  • McCaman, R.E.; Finnerty, W.R.
    Biosynthesis of cytidine diphosphate-diglyceride by a particulate fraction from Micrococcus cerificans (1968), J. Biol. Chem., 243, 5074-5080.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.41 Cutsum enhances activity Micrococcus cerificans
2.7.7.41 non-ionic detergent absolute requirement Micrococcus cerificans
2.7.7.41 Triton X-100 stimulates Micrococcus cerificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.41 hydrazine
-
 Micrococcus cerificans
2.7.7.41 sodium deoxycholate
-
 Micrococcus cerificans
2.7.7.41 sodium lauryl sulfate
-
 Micrococcus cerificans
2.7.7.41 Tween 20
-
 Micrococcus cerificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.41 K+ absolute requirement for Micrococcus cerificans
2.7.7.41 Mg2+ stimulates, 3times more active than Mn2+, optimal activity at 10 mM Micrococcus cerificans
2.7.7.41 Mn2+ stimulates Micrococcus cerificans
2.7.7.41 NH4+ only about half of the activity observed with K+ Micrococcus cerificans
2.7.7.41 Rb+ only about half of the activity observed with K+ Micrococcus cerificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.41 CTP + phosphatidate Micrococcus cerificans enzyme is involved in synthesis of CDP-diglyceride, which plays a primary role in bacterial biosyntheses of essential phosphoglycerides diphosphate + CDPdiacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.41 Micrococcus cerificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.41 partial Micrococcus cerificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.41 0.1333
-
-
 Micrococcus cerificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.41 CTP + phosphatidate highly specific for CTP Micrococcus cerificans diphosphate + CDPdiacylglycerol
-
 ?
2.7.7.41 CTP + phosphatidate enzyme is involved in synthesis of CDP-diglyceride, which plays a primary role in bacterial biosyntheses of essential phosphoglycerides Micrococcus cerificans diphosphate + CDPdiacylglycerol
-
 ?
2.7.7.41 dCTP + phosphatidate 50% of the activity with CTP Micrococcus cerificans diphosphate + dCDPdiacylglycerol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.41 45
-
-
 Micrococcus cerificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.41 55
-
 30 min, irreversible inactivation Micrococcus cerificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.41 8
-
-
 Micrococcus cerificans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.41 6 9.3 pH 6.0: about 45% of maximal activity, pH 9.3: about 40% of maximal activity Micrococcus cerificans