BRENDA - Enzyme Database

Biosynthesis of cytidine diphosphate-diglyceride by a particulate fraction from Micrococcus cerificans

McCaman, R.E.; Finnerty, W.R.; J. Biol. Chem. 243, 5074-5080 (1968)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
Cutsum
enhances activity
Micrococcus cerificans
2.7.7.41
non-ionic detergent
absolute requirement
Micrococcus cerificans
2.7.7.41
Triton X-100
stimulates
Micrococcus cerificans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
hydrazine
-
Micrococcus cerificans
2.7.7.41
sodium deoxycholate
-
Micrococcus cerificans
2.7.7.41
sodium lauryl sulfate
-
Micrococcus cerificans
2.7.7.41
Sulfram
-
Micrococcus cerificans
2.7.7.41
Tween 20
-
Micrococcus cerificans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
K+
absolute requirement for
Micrococcus cerificans
2.7.7.41
Mg2+
stimulates, 3times more active than Mn2+, optimal activity at 10 mM
Micrococcus cerificans
2.7.7.41
Mn2+
stimulates
Micrococcus cerificans
2.7.7.41
NH4+
only about half of the activity observed with K+
Micrococcus cerificans
2.7.7.41
Rb+
only about half of the activity observed with K+
Micrococcus cerificans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.41
CTP + phosphatidate
Micrococcus cerificans
enzyme is involved in synthesis of CDP-diglyceride, which plays a primary role in bacterial biosyntheses of essential phosphoglycerides
diphosphate + CDPdiacylglycerol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Micrococcus cerificans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.41
partial
Micrococcus cerificans
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
0.1333
-
-
Micrococcus cerificans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
highly specific for CTP
643297
Micrococcus cerificans
diphosphate + CDPdiacylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
enzyme is involved in synthesis of CDP-diglyceride, which plays a primary role in bacterial biosyntheses of essential phosphoglycerides
643297
Micrococcus cerificans
diphosphate + CDPdiacylglycerol
-
-
-
?
2.7.7.41
dCTP + phosphatidate
50% of the activity with CTP
643297
Micrococcus cerificans
diphosphate + dCDPdiacylglycerol
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.7.41
45
-
-
Micrococcus cerificans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.7.41
55
-
30 min, irreversible inactivation
Micrococcus cerificans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
8
-
-
Micrococcus cerificans
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.41
6
9.3
pH 6.0: about 45% of maximal activity, pH 9.3: about 40% of maximal activity
Micrococcus cerificans
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
Cutsum
enhances activity
Micrococcus cerificans
2.7.7.41
non-ionic detergent
absolute requirement
Micrococcus cerificans
2.7.7.41
Triton X-100
stimulates
Micrococcus cerificans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
hydrazine
-
Micrococcus cerificans
2.7.7.41
sodium deoxycholate
-
Micrococcus cerificans
2.7.7.41
sodium lauryl sulfate
-
Micrococcus cerificans
2.7.7.41
Sulfram
-
Micrococcus cerificans
2.7.7.41
Tween 20
-
Micrococcus cerificans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
K+
absolute requirement for
Micrococcus cerificans
2.7.7.41
Mg2+
stimulates, 3times more active than Mn2+, optimal activity at 10 mM
Micrococcus cerificans
2.7.7.41
Mn2+
stimulates
Micrococcus cerificans
2.7.7.41
NH4+
only about half of the activity observed with K+
Micrococcus cerificans
2.7.7.41
Rb+
only about half of the activity observed with K+
Micrococcus cerificans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.41
CTP + phosphatidate
Micrococcus cerificans
enzyme is involved in synthesis of CDP-diglyceride, which plays a primary role in bacterial biosyntheses of essential phosphoglycerides
diphosphate + CDPdiacylglycerol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.41
partial
Micrococcus cerificans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
0.1333
-
-
Micrococcus cerificans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
highly specific for CTP
643297
Micrococcus cerificans
diphosphate + CDPdiacylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
enzyme is involved in synthesis of CDP-diglyceride, which plays a primary role in bacterial biosyntheses of essential phosphoglycerides
643297
Micrococcus cerificans
diphosphate + CDPdiacylglycerol
-
-
-
?
2.7.7.41
dCTP + phosphatidate
50% of the activity with CTP
643297
Micrococcus cerificans
diphosphate + dCDPdiacylglycerol
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.7.41
45
-
-
Micrococcus cerificans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.7.41
55
-
30 min, irreversible inactivation
Micrococcus cerificans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
8
-
-
Micrococcus cerificans
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.41
6
9.3
pH 6.0: about 45% of maximal activity, pH 9.3: about 40% of maximal activity
Micrococcus cerificans