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Literature summary extracted from

  • Renosto, F.; Patel, H.C.; Martin, R.L.; Thomassian, C.; Zimmerman, G.; Segel, I.H.
    ATP sulfurylase from higher plants: kinetic and structural characterization of the chloroplast and cytosol enzymes from spinach leaf (1993), Arch. Biochem. Biophys., 307, 272-285.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.4 3',5'-adenosine diphosphate
-
 Spinacia oleracea
2.7.7.4 3'-phosphoadenosine-5'-phosphate
-
 Spinacia oleracea
2.7.7.4 adenosine 5'-monosulfate
-
 Spinacia oleracea
2.7.7.4 adenosine 5'-phosphoramidate
-
 Spinacia oleracea
2.7.7.4 adenosine 5'-phosphosulfate inhibits molybdolysis Spinacia oleracea
2.7.7.4 ADP
-
 Spinacia oleracea
2.7.7.4 AMP
-
 Spinacia oleracea
2.7.7.4 beta-fluoro-adenosine 5'-phosphosulfate
-
 Spinacia oleracea
2.7.7.4 beta-methylene-adenosine 5'-phosphosulfate
-
 Spinacia oleracea
2.7.7.4 NAD+
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.00053
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C, cytosolic enzyme Spinacia oleracea
2.7.7.4 0.0037
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C, chloroplastic enzyme Spinacia oleracea
2.7.7.4 0.017
-
 diphosphate pH 8.0, 30°C, cytosolic enzyme Spinacia oleracea
2.7.7.4 0.045
-
 ATP pH 8.0, 30°C, chloroplastic enzyme, reaction with MoO42- Spinacia oleracea
2.7.7.4 0.045
-
 ATP ATP in form of MgATP2- Spinacia oleracea
2.7.7.4 0.046
-
 ATP pH 8.0, 30°C, chloroplastic enzyme, reaction with SO42- Spinacia oleracea
2.7.7.4 0.046
-
 ATP ATP in form of MgATP2- Spinacia oleracea
2.7.7.4 0.1
-
 diphosphate pH 8.0, 30°C, chloroplastic enzyme Spinacia oleracea
2.7.7.4 0.15
-
 ATP pH 8.0, 30°C, cytosolic enzyme, reaction with MoO42- Spinacia oleracea
2.7.7.4 0.15
-
 ATP ATP in form of MgATP2- Spinacia oleracea
2.7.7.4 0.24
-
 ATP pH 8.0, 30°C, cytosolic enzyme Spinacia oleracea
2.7.7.4 0.24
-
 ATP ATP in form of MgATP2- Spinacia oleracea
2.7.7.4 0.25
-
 SO42- pH 8.0, 30°C, chloroplastic enzyme, reaction with SO42- Spinacia oleracea
2.7.7.4 0.32
-
 MoO42- pH 8.0, 30°C, chloroplastic enzyme Spinacia oleracea
2.7.7.4 0.36
-
 MoO42- pH 8.0, 30°C, cytosolic enzyme Spinacia oleracea
2.7.7.4 0.5
-
 ATP pH 8.0, 30°C, chloroplastic enzym, reaction with FPO32- Spinacia oleracea
2.7.7.4 0.5
-
 ATP ATP in form of MgATP2- Spinacia oleracea
2.7.7.4 0.87
-
 SO42- cytosolic enzyme Spinacia oleracea
2.7.7.4 0.87
-
 SO42- pH 8.0, 30°C Spinacia oleracea
2.7.7.4 1.3
-
 FPO32- pH 8.0, 30°C, chloroplastic enzyme Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.4 chloroplast
-
 Spinacia oleracea 9507
-
2.7.7.4 cytosol
-
 Spinacia oleracea 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 49000
-
 4 * 49000, chloroplastic enzyme, SDS-PAGE Spinacia oleracea
2.7.7.4 50000
-
 4 * 50000, cytosolic enzyme, SDS-PAGE Spinacia oleracea
2.7.7.4 170000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4 a chloroplastic and a cytosolic enzyme form Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.4 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 247
-
 cytosolic enzyme Spinacia oleracea
2.7.7.4 267
-
 chloroplastic enzyme Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + fluorophosphate
-
 Spinacia oleracea diphosphate + adenylyl fluorophosphate
-
 r
2.7.7.4 ATP + FPO32-
-
 Spinacia oleracea ?
-
 ?
2.7.7.4 ATP + molybdate
-
 Spinacia oleracea diphosphate + adenylyl molybdate
-
 r
2.7.7.4 ATP + MoO42-
-
 Spinacia oleracea AMP + adenylylmolybdate
-
 ?
2.7.7.4 ATP + sulfate
-
 Spinacia oleracea diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate
-
 Spinacia oleracea diphosphate + adenylyl sulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.4 tetramer 4 * 49000, chloroplastic enzyme, SDS-PAGE Spinacia oleracea
2.7.7.4 tetramer 4 * 50000, cytosolic enzyme, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.4 8
-
-
 Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.4 7.2 8.5 about 90% of maximal activity at pH 7.2 and pH 8.5 Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.4 0.00007
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C, molybdolysis Spinacia oleracea
2.7.7.4 0.0025
-
 beta-methylene-adenosine 5'-phosphosulfate pH 8.0, 30°C, ATP synthesis Spinacia oleracea
2.7.7.4 0.003
-
 beta-methylene-adenosine 5'-phosphosulfate pH 8.0, 30°C, molybdolysis Spinacia oleracea
2.7.7.4 0.0047
-
 beta-fluoro-adenosine 5'-phosphosulfate pH 8.0, 30°C, synthesis of ATP Spinacia oleracea
2.7.7.4 0.005
-
 beta-fluoro-adenosine 5'-phosphosulfate pH 8.0, 30°C, molybdolysis Spinacia oleracea
2.7.7.4 0.087
-
 ADP pH 8.0, 30°C, synthesis of ATP Spinacia oleracea
2.7.7.4 0.165
-
 AMP pH 8.0, 30°C, synthesis of AMP Spinacia oleracea
2.7.7.4 0.24
-
 adenosine 5'-phosphoramidate pH 8.0, 30°C, synthesis of AMP Spinacia oleracea
2.7.7.4 0.25
-
 adenosine 5'-monosulfate pH 8.0, 30°C, molybdolysis Spinacia oleracea
2.7.7.4 0.35
-
 3',5'-adenosine diphosphate pH 8.0, 30°C, synthesis of ATP Spinacia oleracea
2.7.7.4 0.42
-
 adenosine 5'-monosulfate pH 8.0, 30°C, synthesis of ATP Spinacia oleracea
2.7.7.4 0.9
-
 NAD+ pH 8.0, 30°C, synthesis of ATP Spinacia oleracea
2.7.7.4 1.3
-
 3',5'-adenosine diphosphate pH 8.0, 30°C, molybdolysis Spinacia oleracea
2.7.7.4 2.5
-
 NAD+ pH 8.0, 30°C, molybdolysis Spinacia oleracea