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Literature summary extracted from

  • Li, J.; Saidha, T.; Schiff, J.A.
    Purification and properties of two forms of ATP sulfurylase from Euglena (1991), Biochim. Biophys. Acta, 1078, 68-76.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.001
-
 diphosphate pH 8.8, enzyme form ATPSm Euglena gracilis
2.7.7.4 1
-
 diphosphate pH 8.8, enzyme form ATPSc Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.4 mitochondrial membrane enzyme form ATPSm is mainly associated with mitochondrial membrane Euglena gracilis 31966
-
2.7.7.4 soluble enzyme form ATPSc Euglena gracilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 52300
-
 1 * 52300, enzyme form ATPSc, SDS-PAGE Euglena gracilis
2.7.7.4 55000
-
 1 * 55000, enzyme form ATPSm, SDS-PAGE Euglena gracilis
2.7.7.4 66000
-
 enzyme ATPSm and ATPSc, gel filtration Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Euglena gracilis
-
 Klebs var. bacillaris Cori, aplastidic mutant W10BSmL
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4 enzyme form ATPSm and ATPSc Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 205.2
-
 enzyme form ATPSc Euglena gracilis
2.7.7.4 237.7
-
 enzyme form ATPSm Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + sulfate
-
 Euglena gracilis diphosphate + adenylylsulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.4 monomer 1 * 52300, enzyme form ATPSc, SDS-PAGE Euglena gracilis
2.7.7.4 monomer 1 * 55000, enzyme form ATPSm, SDS-PAGE Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.4 7.3
-
 enzyme form ATPSc, incorporation of SO42- Euglena gracilis
2.7.7.4 8.8
-
 and a second but lower optimum at pH 7.3, enzyme form ATPSm, incorporation of SO42- Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.4 7 7.9 pH 7.0: about 70% of maximal activity, pH 7.0: about 65% of maximal activity, enzyme form ATPSc, incorporation of SO42- Euglena gracilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.4 Euglena gracilis isoelectric focusing, enzyme form ATPSm
-
 5.8
2.7.7.4 Euglena gracilis isoelectric focusing, enzyme form ATPSc
-
 7.9