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Literature summary extracted from

  • Geller, D.H.; Henry, J.G.; Belch, J.; Schwartz, N.B.
    Co-purification and characterization of ATP-sulfurylase and adenosine-5-phosphosulfate kinase from rat chondrosarcoma (1987), J. Biol. Chem., 262, 7374-7382.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.4 Hg2+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.00162
-
 adenosine 5'-phosphosulfate
-
 Rattus norvegicus
2.7.7.4 0.0389
-
 diphosphate pH 7.8, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.4 Ca2+ 50-60% of the activity with Mg2+ Rattus norvegicus
2.7.7.4 Co2+ 50-60% of the activity with Mg2+ Rattus norvegicus
2.7.7.4 Mg2+ Mg2+ is the most efficient divalent cation, Km: 0.122 mM Rattus norvegicus
2.7.7.4 Mn2+ 84% of the activity with Mg2+ Rattus norvegicus
2.7.7.4 Zn2+ 50-60% of the activity with Mg2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 160000
-
 non-denaturing PAGE Rattus norvegicus
2.7.7.4 260000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.4 chondrosarcoma cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 2.953
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + sulfate
-
 Rattus norvegicus diphosphate + adenylylsulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.4 ? x * 35000-37000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.4 46
-
 bimodal temperature optima: 46°C and 52-54°C Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.4 35 60 35°C: about 40% of maximal activity, 60°C: about 35% of maximal activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.4 7 7.4
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.4 7 7.4
-
 Rattus norvegicus