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Literature summary extracted from

  • Burnell, J.N.; Roy, A.B.
    Purification and properties of the ATP sulphurylase of rat liver (1978), Biochim. Biophys. Acta, 527, 239-248.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.4 5°C, enzyme solution with 5-10 mg/ml, stable for several months Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.4 ADP linear competitive inhibitor with respect to SO42-, uncompetitive with respect to ATP Rattus norvegicus
2.7.7.4 AMP linear competitive inhibitor with respect to SO42-, uncompetitive with respect to ATP Rattus norvegicus
2.7.7.4 ATP product inhibitor in formation of ATP from diphosphate and adenylylsulfate Rattus norvegicus
2.7.7.4 ClO3-
-
 Rattus norvegicus
2.7.7.4 ClO4- linear competitive inhibitor with respect to SO42- and uncompetitive with respect to ATP Rattus norvegicus
2.7.7.4 EDTA inhibits due to chelation of Mg2+ Rattus norvegicus
2.7.7.4 NEM 10 mM, inhibits reaction with diphosphate and adenylylsulfate Rattus norvegicus
2.7.7.4 NO3- linear competitive inhibitor with respect to SO42- and uncompetitive with respect to ATP Rattus norvegicus
2.7.7.4 PCMB 5 mM, inhibits reaction with diphosphate and adenylylsulfate Rattus norvegicus
2.7.7.4 SeO42-
-
 Rattus norvegicus
2.7.7.4 SO42- product inhibitor in formation of ATP from diphosphate and adenylylsulfate Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.018
-
 diphosphate pH 7.8, 37°C Rattus norvegicus
2.7.7.4 0.025
-
 adenylylsulfate pH 7.8, 37°C Rattus norvegicus
2.7.7.4 0.38
-
 ATP pH 7.8, 37°C Rattus norvegicus
2.7.7.4 0.61
-
 SeO42- pH 7.8, 37°C, SeO42–dependent ATP-diphosphate exchange reaction Rattus norvegicus
2.7.7.4 2.5
-
 SO42- pH 7.8, 37°C, exchange reaction Rattus norvegicus
2.7.7.4 3.2
-
 SO42- pH 7.8, 37°C, formation of adenylylsulfate Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.4 Mg2+ formation of ATP from diphosphate and adenylylsulfate is absolutely dependent upon the presence of Mg2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 410000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Rattus norvegicus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.7.7.4 not stable to freezing and thawing Rattus norvegicus

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.7.4 lipoprotein
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.4 ATP + sulfate = diphosphate + adenylyl sulfate sequential bi-bi reaction with ATP being the first substrate bound and adenylysulfate the last product released Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.4 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 2.1
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + SeO42-
-
 Rattus norvegicus AMP + adenylylselenate
-
 ?
2.7.7.4 ATP + sulfate
-
 Rattus norvegicus diphosphate + adenylylsulfate
-
 r
2.7.7.4 additional information enzyme catalyzes ATP-diphosphate exchange reaction. The enzyme does notto catalyze the incorporation of diphosphate into ATP in the absence of SO42-. The enzyme catalyzes SeO42–dependent ATP-diphosphate exchange Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.4 7.8 8 0.1 M phosphate buffer Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.4 7.8
-
 unstable in Tris, diethylbarbiturate, glycine, triethanolamine and bicarbonate buffer, but stable in phosphate buffers of the same pH Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.4 0.06
-
 ADP pH 7.8, 37°C Rattus norvegicus
2.7.7.4 0.25
-
 ClO3- pH 7.8, 37°C Rattus norvegicus
2.7.7.4 0.5
-
 SeO42- pH 7.8, 37°C Rattus norvegicus
2.7.7.4 0.6
-
 AMP pH 7.8, 37°C Rattus norvegicus
2.7.7.4 2.2
-
 NO3- pH 7.8, 37°C Rattus norvegicus