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Literature summary extracted from

  • Evans, W.R.
    Adenosine diphosphoribose phosphorylase from Euglena gracilis (1971), Methods Enzymol., 23A, 566-570.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.35 AMP
-
 Euglena gracilis
2.7.7.35 arsenate
-
 Euglena gracilis
2.7.7.35 ATP
-
 Euglena gracilis
2.7.7.35 D-ribose 5-phosphate
-
 Euglena gracilis
2.7.7.35 additional information
-
 Euglena gracilis
2.7.7.35 PCMB ADP/phosphate-exchange and ADPribose phosphorolysis Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.35 0.04
-
 ADP-ribose pH 7.8, 30°C Euglena gracilis
2.7.7.35 0.4
-
 phosphate pH 7.8, 30°C Euglena gracilis
2.7.7.35 0.6
-
 ADP ADP/phosphate exchange Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.35 cytosol
-
 Euglena gracilis 5829
-
2.7.7.35 cytosol
-
 Ochromonas danica 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.35 additional information no metal ion requirement Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.35 phosphate + ADP-ribose Euglena gracilis
-
 ADP + D-ribose 5-phosphate
-
 ?
2.7.7.35 phosphate + ADP-ribose Ochromonas danica
-
 ADP + D-ribose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.35 Euglena gracilis
-
 green alga, strain Z
-
2.7.7.35 Ochromonas danica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.35 partial Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.35 cell culture
-
 Ochromonas danica
-
2.7.7.35 cell culture heterotrophically or autotrophically grown cells Euglena gracilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.35 additional information
-
 low activity Ochromonas danica
2.7.7.35 1.3
-
 partially purified enzyme Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.35 phosphate + ADP-ribose
-
 Euglena gracilis ADP + D-ribose 5-phosphate
-
 ?
2.7.7.35 phosphate + ADP-ribose
-
 Ochromonas danica ADP + D-ribose 5-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.35 7.8 8
-
 Euglena gracilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.35 0.09
-
 D-ribose 5-phosphate phosphorolysis Euglena gracilis
2.7.7.35 0.09
-
 D-ribose 5-phosphate pH 7.8, 30°C Euglena gracilis
2.7.7.35 0.11
-
 D-ribose 5-phosphate pH 7.8, 30°C Euglena gracilis
2.7.7.35 0.11
-
 D-ribose 5-phosphate ADP/phosphate-exchange reaction Euglena gracilis
2.7.7.35 0.41
-
 arsenate pH 7.8, 30°C Euglena gracilis
2.7.7.35 0.41
-
 arsenate ADP/phosphate-exchange reaction Euglena gracilis
2.7.7.35 0.57
-
 arsenate phosphorolysis Euglena gracilis
2.7.7.35 0.57
-
 arsenate pH 7.8, 30°C Euglena gracilis
2.7.7.35 1
-
 AMP phosphorolysis Euglena gracilis
2.7.7.35 1
-
 AMP pH 7.8, 30°C Euglena gracilis
2.7.7.35 1.3
-
 ATP pH 7.8, 30°C Euglena gracilis
2.7.7.35 1.3
-
 ATP ADP/phosphate-exchange reaction Euglena gracilis
2.7.7.35 1.5
-
 AMP pH 7.8, 30°C Euglena gracilis
2.7.7.35 1.5
-
 AMP ADP/phosphate-exchange reaction Euglena gracilis
2.7.7.35 2.9
-
 ATP phosphorolysis Euglena gracilis
2.7.7.35 2.9
-
 ATP pH 7.8, 30°C Euglena gracilis