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Literature summary extracted from

  • Lindqvist, L.; Kaiser, R.; Reeves, P.R.; Lindberg, A.A.
    Purification, characterization, and high performance liquid chromatography assay of Salmonella glucose-1-phosphate cytidylyltransferase from the cloned rfbF gene (1994), J. Biol. Chem., 269, 122-126.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.33
-
 Salmonella enterica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.33 CDPglucose product inhibition, competitive with respect to CTP, kinetics Salmonella enterica
2.7.7.33 diphosphate product inhibition, kinetics Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.33 0.11
-
 CTP pH 8, 37°C Salmonella enterica
2.7.7.33 0.28
-
 CTP pH 8, 37°C Salmonella enterica
2.7.7.33 0.64
-
 alpha-D-glucose 1-phosphate pH 8, 37°C Salmonella enterica
2.7.7.33 1.89
-
 diphosphate pH 8, 37°C Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.33 29035
-
 x * 29035, calculated from amino acid composition deduced from nucleotide sequence, x * 31000, SDS-PAGE Salmonella enterica
2.7.7.33 31000
-
 x * 29035, calculated from amino acid composition deduced from nucleotide sequence, x * 31000, SDS-PAGE Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.33 CTP + D-glucose 1-phosphate Salmonella enterica first of five enzymes committed to CDP-D-abequose biosynthesis ?
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.33 Salmonella enterica
-
 LT2, ecombinant, overproducing strain P9254
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.33 10.3
-
-
 Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.33 CTP + alpha-D-glucose 1-phosphate best substrates Salmonella enterica diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrates in the reverse reaction: ADPglucose, dTDPglucose, UDPglucose Salmonella enterica diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: alpha-D-mannose 1-phosphate, N-acetyl-alpha-D-glucosamine 1-phosphate Salmonella enterica diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: dTTP, alpha-D-galactose 1-phosphate Salmonella enterica diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: ATP, GTP Salmonella enterica diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate catalyzes bimolecular group transfer reaction Salmonella enterica diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrates in the reverse reaction: UDPgalactose, UDP-D-mannose, UDP-N-acetyl-D-glucosamine, dTDP-L-rhamnose Salmonella enterica diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: D-ribose 1-phosphate Salmonella enterica diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-xylose 1-phosphate poor substrate Salmonella enterica diphosphate + CDPxylose
-
 ?
2.7.7.33 CTP + D-glucosamine 1-phosphate reaction at about 15% the rate with glucose 1-phosphate Salmonella enterica diphosphate + CDPglucosamine
-
 ?
2.7.7.33 CTP + D-glucose 1-phosphate first of five enzymes committed to CDP-D-abequose biosynthesis Salmonella enterica ?
-
 r
2.7.7.33 UTP + alpha-D-glucose 1-phosphate poor substrate Salmonella enterica diphosphate + UDP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.33 ? x * 29035, calculated from amino acid composition deduced from nucleotide sequence, x * 31000, SDS-PAGE Salmonella enterica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.33 37
-
 assay at Salmonella enterica