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Literature summary extracted from

  • Kimata, K.; Suzuki, S.
    Studies on cytidine diphosphate glucose pyrophosphorylase and related enzymes of Azotobacter vinelandii (1966), J. Biol. Chem., 241, 1099-1113.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.33 additional information no activation by dTDP-L-rhamnose, dTDP-4-amino-4,6-dideoxy-D-glucose and its N-acetyl-derivative, UDP-D-galactose, UDP-D-glucuronic acid, UDP-N-acetyl-D-glucosamine, GDP-D-mannose, D-glucose 6-phosphate, D-glucose 1,6-diphosphate, D-galactose 1-phosphate, D-glucosamine 1-phosphate, D-mannose 1-phosphate, N-acetyl-D-glucosamine 1-phosphate, D-fructose 6-phosphate, D-fructose 1,6-diphosphate, D-ribitol 5-phosphate, L-glycerol 3-phosphate, phosphoenolpyruvate, pyruvate, ADP, AMP, GMP, UMP, beta-NAD+ or beta-NADP+ Azotobacter vinelandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.33 ADPglucose weak Azotobacter vinelandii
2.7.7.33 ATP
-
Azotobacter vinelandii
2.7.7.33 CDP weak Azotobacter vinelandii
2.7.7.33 CDP-2-O-methyldeoxyaldose
-
Azotobacter vinelandii
2.7.7.33 CDP-D-galactose weak Azotobacter vinelandii
2.7.7.33 CDPglucose product inhibition, competitive with respect to CTP, kinetics Azotobacter vinelandii
2.7.7.33 dADP weak Azotobacter vinelandii
2.7.7.33 dATP
-
Azotobacter vinelandii
2.7.7.33 dGTP
-
Azotobacter vinelandii
2.7.7.33 diphosphate product inhibition, kinetics Azotobacter vinelandii
2.7.7.33 dTDP weak Azotobacter vinelandii
2.7.7.33 dTTP strong, competitive with CTP, kinetics Azotobacter vinelandii
2.7.7.33 GTP
-
Azotobacter vinelandii
2.7.7.33 additional information little or no inhibition by CDPethanolamine, CDPcholine, dTTPglucose, UDP-D-glucosamine, dCTP, dCDP, dTMP, dAMP, dGMP, dCMP, dTDP-L-rhamnose, dTDP-4-amino-4,6-dideoxy-D-glucose and its N-acetyl-derivative, UDP-D-galactose, UDP-D-glucuronic acid, UDP-N-acetyl-D-glucosamine, GDP-D-mannose, D-glucose 6-phosphate, D-glucose 1,6-diphosphate, D-galactose 1-phosphate, D-glucosamine 1-phosphate, D-mannose 1-phosphate, N-acetyl-D-glucosamine 1-phosphate, D-fructose 6-phosphate, D-fructose 1,6-diphosphate, D-ribitol 5-phosphate, L-glycerol 3-phosphate, phosphoenolpyruvate, pyruvate, ADP, AMP, GMP, UMP, beta-NAD+ or beta-NADP+; little or no inhibition by CMP, GDPglucose, UDPglucose, dTDPglucose Azotobacter vinelandii
2.7.7.33 phosphate weak Azotobacter vinelandii
2.7.7.33 UTP
-
Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.33 0.56
-
diphosphate pH 8.5, 37°C Azotobacter vinelandii
2.7.7.33 0.56
-
diphosphate diphosphate in form of magnesium diphosphate Azotobacter vinelandii
2.7.7.33 5
-
D-glucosamine 1-phosphate pH 8.5, 37°C Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.33 Mg2+ requirement Azotobacter vinelandii
2.7.7.33 Mg2+ maximal reaction rates at molar ratios of MgCl2:diphosphate or MgCl2:CTP of 2:1 Azotobacter vinelandii
2.7.7.33 Mn2+ requirement Azotobacter vinelandii
2.7.7.33 Mn2+ can replace Mg2+ with 87% efficiency Azotobacter vinelandii
2.7.7.33 additional information no activation by Ni2+ Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.33 Azotobacter vinelandii
-
-
-
2.7.7.33 Azotobacter vinelandii O
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.33 partial Azotobacter vinelandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.33 0.0375
-
-
Azotobacter vinelandii

Storage Stability

EC Number Storage Stability Organism
2.7.7.33 -18°C, in EDTA-containing Tris-HCl buffer, at least 1 year Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.33 CTP + alpha-D-glucose 1-phosphate highly specific Azotobacter vinelandii diphosphate + CDPglucose
-
r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrates in the reverse reaction: ADPglucose, dTDPglucose, UDPglucose Azotobacter vinelandii diphosphate + CDPglucose
-
r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: alpha-D-mannose 1-phosphate, N-acetyl-alpha-D-glucosamine 1-phosphate Azotobacter vinelandii diphosphate + CDPglucose
-
r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: dTTP, alpha-D-galactose 1-phosphate Azotobacter vinelandii diphosphate + CDPglucose
-
r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: ATP, GTP Azotobacter vinelandii diphosphate + CDPglucose
-
r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: dATP, dGTP, dCTP, D-ribitol 5-phosphate, L-glycerol 3-phosphate Azotobacter vinelandii diphosphate + CDPglucose
-
r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrates in the reverse reaction: GDPglucose Azotobacter vinelandii diphosphate + CDPglucose
-
r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate highly specific Azotobacter vinelandii O diphosphate + CDPglucose
-
r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrates in the reverse reaction: ADPglucose, dTDPglucose, UDPglucose Azotobacter vinelandii O diphosphate + CDPglucose
-
r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: alpha-D-mannose 1-phosphate, N-acetyl-alpha-D-glucosamine 1-phosphate Azotobacter vinelandii O diphosphate + CDPglucose
-
r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: dTTP, alpha-D-galactose 1-phosphate Azotobacter vinelandii O diphosphate + CDPglucose
-
r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: ATP, GTP Azotobacter vinelandii O diphosphate + CDPglucose
-
r
2.7.7.33 CTP + D-glucosamine 1-phosphate reaction at about 25% the rate with glucose 1-phosphate Azotobacter vinelandii diphosphate + CDPglucosamine
-
?
2.7.7.33 diphosphate + CDP-glucose
-
Azotobacter vinelandii CTP + alpha-D-glucose 1-phosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.33 37
-
assay at Azotobacter vinelandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.33 8.3
-
diphosphorolysis Azotobacter vinelandii
2.7.7.33 8.5
-
CDPglucose synthesis Azotobacter vinelandii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.33 0.04
-
dTTP pH 8.5, 37°C Azotobacter vinelandii
2.7.7.33 0.3
-
diphosphate pH 8.5, 37°C Azotobacter vinelandii
2.7.7.33 0.35
-
CDPglucose pH 8.5, 37°C Azotobacter vinelandii