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Literature summary extracted from

  • Kaneda, T.; Kuroda, S.; Koiwai, O.; Yoshida, S.
    Purification of terminal deoxynucleotidyl transferase from pig thymus: identification of 42,000 and 57,000 dalton species (1981), J. Biochem., 90, 1421-1427.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.31 ATP
-
 Sus scrofa
2.7.7.31 N-ethylmaleimide
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.31 0.02
-
 oligo(dA)12-18 37°C, pH 8.3 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.31 42000
-
 1 * 42000, low-MW form, SDS-PAGE Sus scrofa
2.7.7.31 45000
-
 2 MW forms: 45000 and 57000, gel filtration Sus scrofa
2.7.7.31 57000
-
 2 MW forms: 45000 and 57000, gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.31 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.31 2 MW forms: 45000 and 57000 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.31 additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.31 deoxynucleoside triphosphate + DNAn primer required Sus scrofa diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNAn reaction is not template directed Sus scrofa diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNAn deoxynucleoside triphosphate preference: dGTP, dCTP, dTTP, dATP (42000 MW enzyme) Sus scrofa diphosphate + DNAn+1
-
 ?
2.7.7.31 dGTP + oligo(dA)12-18
-
 Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.31 monomer 1 * 57000, high-MW form, SDS-PAGE Sus scrofa
2.7.7.31 monomer 1 * 42000, low-MW form, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.31 37
-
 assay at Sus scrofa