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Literature summary extracted from

  • Bollum, F.J.
    Terminal deoxyribonucleotidyl transferase (1974), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 10, 145-171.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.7.31 not stable in urea, SDS and organic solvents Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.31 1,10-phenanthroline not m-isomer Bos taurus
2.7.7.31 2,2'-dipyridyl
-
 Bos taurus
2.7.7.31 cysteine
-
 Bos taurus
2.7.7.31 EDTA
-
 Bos taurus
2.7.7.31 high ionic strength
-
 Bos taurus
2.7.7.31 histidine
-
 Bos taurus
2.7.7.31 Mercaptoacetate
-
 Bos taurus
2.7.7.31 metal chelators
-
 Bos taurus
2.7.7.31 triethylenetetramine
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.31 additional information
-
 additional information overview, Km-values for dATP polymerization with various primers Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.31 Mg2+ Mg2+ or Co2+ Bos taurus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.7.31 additional information not stable in organic solvents Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.31 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.31 thymus
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
2.7.7.31 -20°C, 50 mM potassium phosphate buffer, pH 7.0, stable for 9 years Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.31 dATP + DNAn
-
 Bos taurus diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNAn primer required Bos taurus diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn reaction is not template directed Bos taurus diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn relative rates of polymerization with several nucleotides Bos taurus diphosphate + DNAn+1
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.31 35
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.31 40
-
 not stable above Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.31 0.833
-
 dATP
-
 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.31 4.5
-
 stable at Bos taurus