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Literature summary extracted from

  • Ratliff, R.L.
    Terminal deoxyribonucleotidyl transferase (1981), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 14, 105-118.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.7.31 not stable in urea, SDS and organic solvents Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.31 Co2+ Co2+ is the best activator in elongation of chains with dCTP or dTTP Mus musculus
2.7.7.31 Co2+ Co2+ is the best activator in elongation of chains with dCTP or dTTP Homo sapiens
2.7.7.31 Co2+ Co2+ is the best activator in elongation of chains with dCTP or dTTP Bos taurus
2.7.7.31 Mg2+ activates Mus musculus
2.7.7.31 Mg2+ activates Homo sapiens
2.7.7.31 Mg2+ activates Bos taurus
2.7.7.31 Mn2+
-
 Mus musculus
2.7.7.31 Mn2+
-
 Bos taurus
2.7.7.31 Mn2+ human enzyme from leukemic cells shows maximal activity with Mn2+ as divalent cation Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.31 60000
-
-
 Mus musculus
2.7.7.31 60000
-
-
 Homo sapiens
2.7.7.31 79000
-
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.31 deoxynucleoside triphosphate + DNA Mus musculus hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNA Homo sapiens hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNA Bos taurus hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides diphosphate + DNAn+1
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.7.31 additional information not stable in organic solvents Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.31 Bos taurus
-
-
-
2.7.7.31 Homo sapiens
-
-
-
2.7.7.31 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.31
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.31 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 mechanism Mus musculus
a
2.7.7.31 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 mechanism Homo sapiens
a
2.7.7.31 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.31 bone marrow
-
 Mus musculus
-
2.7.7.31 bone marrow
-
 Homo sapiens
-
2.7.7.31 bone marrow
-
 Bos taurus
-
2.7.7.31 thymus
-
 Mus musculus
-
2.7.7.31 thymus
-
 Homo sapiens
-
2.7.7.31 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.31 additional information
-
 overview assay methods Mus musculus
2.7.7.31 additional information
-
 overview assay methods Homo sapiens
2.7.7.31 additional information
-
 overview assay methods Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.31 dATP + DNAn
-
 Bos taurus diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNA hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides Mus musculus diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNA hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNA hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides Bos taurus diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNAn primer required Mus musculus diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn primer required Homo sapiens diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn primer required Bos taurus diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn longer chains interact well with the enzyme, but synthesis rate decreases Mus musculus diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn longer chains interact well with the enzyme, but synthesis rate decreases Homo sapiens diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn longer chains interact well with the enzyme, but synthesis rate decreases Bos taurus diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn primer: oligonucleotides containing at least 3 phosphate groups and a free 3'-hydroxyl end Mus musculus diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn primer: oligonucleotides containing at least 3 phosphate groups and a free 3'-hydroxyl end Homo sapiens diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn primer: oligonucleotides containing at least 3 phosphate groups and a free 3'-hydroxyl end Bos taurus diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn reaction is not template directed Mus musculus diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn reaction is not template directed Homo sapiens diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn reaction is not template directed Bos taurus diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn as the initiator chain length is increased to 5-7 nucleotides the polymerization rate also increases Mus musculus diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn as the initiator chain length is increased to 5-7 nucleotides the polymerization rate also increases Homo sapiens diphosphate + DNAn+1
-
 ir
2.7.7.31 deoxynucleoside triphosphate + DNAn as the initiator chain length is increased to 5-7 nucleotides the polymerization rate also increases Bos taurus diphosphate + DNAn+1
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.7.31 monomer
-
 Homo sapiens
2.7.7.31 monomer 1 * 60000 Mus musculus
2.7.7.31 monomer 1 * 79000 Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.31 40
-
 not stable above Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.31 0.833
-
 dATP
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.31 7.2
-
-
 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.31 4.5
-
 stable at Bos taurus