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Literature summary extracted from

  • Ko, J.H.; Kim, C.H.; Lee, D.S.; Kim, Y.S.
    Purification and characterization of a thermostable ADP-glucose pyrophosphorylase from Thermus caldophilus GK-24 (1996), Biochem. J., 319, 977-983.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate
-
 Escherichia coli
2.7.7.27 3-phosphoglycerate
-
 Cereibacter sphaeroides
2.7.7.27 3-phosphoglycerate
-
 Rhodospirillum rubrum
2.7.7.27 3-phosphoglycerate
-
 Anabaena sp.
2.7.7.27 3-phosphoglycerate minimal activation Thermus caldophilus
2.7.7.27 D-fructose 1,6-bisphosphate
-
 Escherichia coli
2.7.7.27 D-fructose 1,6-bisphosphate
-
 Cereibacter sphaeroides
2.7.7.27 D-fructose 1,6-bisphosphate
-
 Rhodospirillum rubrum
2.7.7.27 D-fructose 1,6-bisphosphate
-
 Anabaena sp.
2.7.7.27 D-fructose 1,6-bisphosphate the most effective activator Thermus caldophilus
2.7.7.27 D-fructose 6-phosphate
-
 Escherichia coli
2.7.7.27 D-fructose 6-phosphate
-
 Cereibacter sphaeroides
2.7.7.27 D-fructose 6-phosphate
-
 Rhodospirillum rubrum
2.7.7.27 D-fructose 6-phosphate
-
 Anabaena sp.
2.7.7.27 D-fructose 6-phosphate
-
 Thermus caldophilus
2.7.7.27 D-glucose 6-phosphate
-
 Escherichia coli
2.7.7.27 D-glucose 6-phosphate
-
 Thermus caldophilus
2.7.7.27 Phenylglyoxal activation at pH 7 Thermus caldophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27
-
 Thermus caldophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 ADP
-
 Cereibacter sphaeroides
2.7.7.27 ADP
-
 Escherichia coli
2.7.7.27 ADP
-
 Rhodospirillum rubrum
2.7.7.27 ADP
-
 Thermus caldophilus
2.7.7.27 AMP
-
 Cereibacter sphaeroides
2.7.7.27 AMP
-
 Escherichia coli
2.7.7.27 AMP
-
 Rhodospirillum rubrum
2.7.7.27 AMP
-
 Thermus caldophilus
2.7.7.27 phosphate
-
 Anabaena sp.
2.7.7.27 phosphate
-
 Cereibacter sphaeroides
2.7.7.27 phosphate
-
 Escherichia coli
2.7.7.27 phosphate
-
 Rhodospirillum rubrum
2.7.7.27 phosphate
-
 Thermus caldophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 1.9
-
 ATP pH 7.6, 75°C Thermus caldophilus
2.7.7.27 4.2
-
 alpha-D-glucose 1-phosphate pH 7.6, 75°C Thermus caldophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+ requirement Escherichia coli
2.7.7.27 Mg2+ requirement Cereibacter sphaeroides
2.7.7.27 Mg2+ requirement Rhodospirillum rubrum
2.7.7.27 Mg2+ requirement Anabaena sp.
2.7.7.27 Mg2+ requirement Thermus caldophilus
2.7.7.27 additional information Co2+ and Mn2+ are able to replace Mg2+, but not as effective as Mg2+ Thermus caldophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 additional information
-
 amino acid composition and comparison Escherichia coli
2.7.7.27 additional information
-
 amino acid composition and comparison Cereibacter sphaeroides
2.7.7.27 additional information
-
 amino acid composition and comparison Rhodospirillum rubrum
2.7.7.27 additional information
-
 amino acid composition and comparison Anabaena sp.
2.7.7.27 additional information
-
 amino acid composition and comparison Thermus caldophilus
2.7.7.27 52000
-
 4 * 52000, SDS-PAGE Thermus caldophilus
2.7.7.27 230000
-
 gel filtration Thermus caldophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Anabaena sp.
-
-
-
2.7.7.27 Cereibacter sphaeroides
-
-
-
2.7.7.27 Escherichia coli
-
-
-
2.7.7.27 Rhodospirillum rubrum
-
-
-
2.7.7.27 Thermus caldophilus
-
 GK-24
-
2.7.7.27 Thermus caldophilus GK-24
-
 GK-24
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27
-
 Thermus caldophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 161.7
-
-
 Thermus caldophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Escherichia coli diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Cereibacter sphaeroides diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Rhodospirillum rubrum diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Anabaena sp. diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate not: ITP Thermus caldophilus diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate no substrate are GTP, dATP, CTP Thermus caldophilus diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate no substrate is UTP Thermus caldophilus diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate not: ITP Thermus caldophilus GK-24 diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate no substrate are GTP, dATP, CTP Thermus caldophilus GK-24 diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate no substrate is UTP Thermus caldophilus GK-24 diphosphate + ADP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.27 homotetramer
-
 Escherichia coli
2.7.7.27 homotetramer
-
 Cereibacter sphaeroides
2.7.7.27 homotetramer
-
 Rhodospirillum rubrum
2.7.7.27 homotetramer
-
 Anabaena sp.
2.7.7.27 homotetramer 4 * 52000, SDS-PAGE Thermus caldophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.27 73
-
-
 Thermus caldophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.27 95
-
 30 min, 50% loss of activity Thermus caldophilus