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Literature summary extracted from

  • Crevillen, P.; Ballicora, M.A.; Merida, A.; Preiss, J.; Romero, J.M.
    The different large subunit isoforms of Arabidopsis thaliana ADP-glucose pyrophosphorylase confer distinct kinetic and regulatory properties to the heterotetrameric enzyme (2003), J. Biol. Chem., 278, 28508-28515.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate kinetic study and comparison of activation of recombinant enzymes, the activation is more drastic for recombinant enzyme APS1/APL1 Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 six genes coding for enzyme isoforms, two small and four large subunits are cloned and expressed in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 additional information
-
 Hordeum vulgare
2.7.7.27 additional information coexpression of the small subunit APS1 with the different large subunits APL1, APL2, APL3 and APL4, results in heterotetramers with different regulatory and kinetic properties, APS1/APL1 shows the highest affinity for the substrates and the highest sensitivity to the allosteric effectors Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 phosphate kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.019
-
 alpha-D-glucose 1-phosphate pH 7.9, APS1/APL1 in presence of 0.1 mM 3-phosphoglycerate Arabidopsis thaliana
2.7.7.27 0.052
-
 alpha-D-glucose 1-phosphate pH 7.9, APS1/APL3 in presence of 2 mM 3-phosphoglycerate Arabidopsis thaliana
2.7.7.27 0.06
-
 alpha-D-glucose 1-phosphate pH 7.9, APS1/APL4 in presence of 1 mM 3-phosphoglycerate Arabidopsis thaliana
2.7.7.27 0.067
-
 ATP pH 7.9, APS1/APL1 in presence of 0.1 mM 3-phosphoglycerate Arabidopsis thaliana
2.7.7.27 0.076
-
 alpha-D-glucose 1-phosphate pH 7.9, homotetramer APS1 in presence of 20 mM 3-phosphoglycerate Arabidopsis thaliana
2.7.7.27 0.085
-
 alpha-D-glucose 1-phosphate pH 7.9, APS1/APL2 in presence of 4 mM 3-phosphoglycerate Arabidopsis thaliana
2.7.7.27 0.094
-
 ATP pH 7.9, APS1/APL3 in presence of 2 mM 3-phosphoglycerate Arabidopsis thaliana
2.7.7.27 0.118
-
 ATP
-
 Arabidopsis thaliana
2.7.7.27 0.118
-
 ATP pH 7.9, APS1/APL4 in presence of 1 mM 3-phosphoglycerate Arabidopsis thaliana
2.7.7.27 0.42
-
 ATP pH 7.9, homotetramer APS1 in presence of 20 mM 3-phosphoglycerate Arabidopsis thaliana
2.7.7.27 0.57
-
 ATP pH 7.9, APS1/APL2 in presence of 4 mM 3-phosphoglycerate Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 additional information
-
-
 Hordeum vulgare
2.7.7.27 additional information
-
-
 Oryza sativa
2.7.7.27 additional information
-
-
 Solanum tuberosum
2.7.7.27 additional information
-
-
 Arabidopsis thaliana
2.7.7.27 additional information
-
-
 Solanum lycopersicum
2.7.7.27 additional information
-
-
 Triticum aestivum
2.7.7.27 additional information
-
-
 Ipomoea batatas
2.7.7.27 additional information
-
-
 Pisum sativum
2.7.7.27 additional information
-
-
 Beta vulgaris
2.7.7.27 additional information
-
-
 Zea mays
2.7.7.27 additional information
-
-
 Vicia faba
2.7.7.27 additional information
-
 amino acid composition and comparison Oryza sativa
2.7.7.27 additional information
-
 amino acid composition and comparison Synechocystis sp.
2.7.7.27 additional information
-
 amino acid composition and comparison Solanum tuberosum
2.7.7.27 additional information
-
 amino acid composition and comparison Arabidopsis thaliana
2.7.7.27 additional information
-
 amino acid composition and comparison Anabaena sp.
2.7.7.27 additional information
-
 amino acid composition and comparison Solanum lycopersicum
2.7.7.27 additional information
-
 amino acid composition and comparison Hordeum vulgare
2.7.7.27 additional information
-
 amino acid composition and comparison Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Anabaena sp. P30521
-
-
2.7.7.27 Arabidopsis thaliana P55228 APS1
-
2.7.7.27 Arabidopsis thaliana P55229 APL1
-
2.7.7.27 Arabidopsis thaliana P55230 APL2
-
2.7.7.27 Arabidopsis thaliana P55231 APL3
-
2.7.7.27 Arabidopsis thaliana Q9SIK1 APL1
-
2.7.7.27 Beta vulgaris P55232 small subunit
-
2.7.7.27 Beta vulgaris P55233 large subunit
-
2.7.7.27 Hordeum vulgare O04896 barley, large subunit
-
2.7.7.27 Hordeum vulgare P30524 barley, large subunit
-
2.7.7.27 Hordeum vulgare P55238 barley, small subunit
-
2.7.7.27 Ipomoea batatas O81274 sweet potato, small subunit
-
2.7.7.27 Ipomoea batatas Q42859 sweet potato, small subunit
-
2.7.7.27 no activity in Arabidopsis thaliana
-
 subunit APS2
-
2.7.7.27 Oryza sativa O23809 large subunit
-
2.7.7.27 Oryza sativa P15280 small subunit
-
2.7.7.27 Oryza sativa P93430 large subunit
-
2.7.7.27 Oryza sativa Q9ARH9 small subunit
-
2.7.7.27 Pisum sativum Q43815 pea, SS1
-
2.7.7.27 Pisum sativum Q43816 pea, SS2
-
2.7.7.27 Pisum sativum Q43819 pea, large subunit
-
2.7.7.27 Solanum lycopersicum O04924 tomato, LS1
-
2.7.7.27 Solanum lycopersicum P93229 tomato, LS2
-
2.7.7.27 Solanum lycopersicum P93230 tomato, LS3
-
2.7.7.27 Solanum lycopersicum Q42882 tomato, small subunit
-
2.7.7.27 Solanum tuberosum P23509
-
-
2.7.7.27 Solanum tuberosum P55242
-
-
2.7.7.27 Solanum tuberosum P55243 LS3
-
2.7.7.27 Solanum tuberosum Q00081 LS1
-
2.7.7.27 Solanum tuberosum LS1 Q00081 LS1
-
2.7.7.27 Solanum tuberosum LS3 P55243 LS3
-
2.7.7.27 Synechocystis sp. P52415
-
-
2.7.7.27 Triticum aestivum P12299 wheat, large subunit
-
2.7.7.27 Triticum aestivum P30523 wheat, small subunit
-
2.7.7.27 Vicia faba P52416 large subunit
-
2.7.7.27 Vicia faba P52416 SS1
-
2.7.7.27 Vicia faba P52417 SS2
-
2.7.7.27 Vicia faba SS1 P52416 SS1
-
2.7.7.27 Vicia faba SS2 P52417 SS2
-
2.7.7.27 Zea mays Q941P2 small subunit
-
2.7.7.27 Zea mays Q947B9 small subunit
-
2.7.7.27 Zea mays Q947C0 small subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 partial, recombinant enzymes, heat treatment step of heterotetramers formed by small subunit APS1 with any of the large subunits APL1, APL2, APL3 or APL4 at 65°C and of homotetramer APS1 at 70°C, only fractions that show activity at two concentrations of activator 3-phosphoglycerate, 0.5 and 10 mM are pooled Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 embryo
-
 Zea mays
-
2.7.7.27 embryo
-
 Vicia faba
-
2.7.7.27 endosperm
-
 Hordeum vulgare
-
2.7.7.27 endosperm
-
 Oryza sativa
-
2.7.7.27 endosperm
-
 Triticum aestivum
-
2.7.7.27 endosperm
-
 Zea mays
-
2.7.7.27 endosperm
-
 Vicia faba
-
2.7.7.27 fruit
-
 Solanum lycopersicum
-
2.7.7.27 fruit
-
 Beta vulgaris
-
2.7.7.27 leaf
-
 Oryza sativa
-
2.7.7.27 leaf
-
 Solanum tuberosum
-
2.7.7.27 leaf
-
 Arabidopsis thaliana
-
2.7.7.27 leaf
-
 Solanum lycopersicum
-
2.7.7.27 leaf
-
 Hordeum vulgare
-
2.7.7.27 leaf
-
 Ipomoea batatas
-
2.7.7.27 leaf
-
 Pisum sativum
-
2.7.7.27 leaf
-
 Beta vulgaris
-
2.7.7.27 leaf
-
 Zea mays
-
2.7.7.27 leaf
-
 Vicia faba
-
2.7.7.27 leaf sink and source leaves Beta vulgaris
-
2.7.7.27 leaf sink leaves Solanum tuberosum
-
2.7.7.27 root
-
 Solanum lycopersicum
-
2.7.7.27 seed
-
 Oryza sativa
-
2.7.7.27 seed
-
 Pisum sativum
-
2.7.7.27 seed
-
 Vicia faba
-
2.7.7.27 stem
-
 Solanum lycopersicum
-
2.7.7.27 stem
-
 Ipomoea batatas
-
2.7.7.27 tuber
-
 Solanum tuberosum
-
2.7.7.27 tuber
-
 Ipomoea batatas
-
2.7.7.27 vascular tissue
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 2.5
-
 recombinant enzyme APS2 plus APL3 Arabidopsis thaliana
2.7.7.27 3.7
-
 recombinant enzyme APS2 plus APL1 Arabidopsis thaliana
2.7.7.27 5
-
 recombinant enzyme APS1 plus APL2 Arabidopsis thaliana
2.7.7.27 16
-
 recombinant enzyme, homotetramer APS1 Arabidopsis thaliana
2.7.7.27 17
-
 recombinant enzyme APS1 plus APL4 Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
2.7.7.27 -80°C, stable for at least 3 months Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Hordeum vulgare diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Oryza sativa diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Synechocystis sp. diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Arabidopsis thaliana diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Anabaena sp. diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum lycopersicum diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Triticum aestivum diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Ipomoea batatas diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Pisum sativum diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Beta vulgaris diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Zea mays diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Vicia faba diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Vicia faba SS1 diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Vicia faba SS2 diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum LS1 diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum LS3 diphosphate + ADP-glucose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.27 65
-
 purification of heterotetramers formed by small subunit APS1 with any of the large subunits APL1, APL2, APL3, or APL4 involves a 5 min/65°C heat treatment step Arabidopsis thaliana
2.7.7.27 70
-
 purification of homotetramer APS1 involves a 5 min/70°C heat treatment step Arabidopsis thaliana