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Literature summary extracted from

  • Ballicora, M.A.; Laughlin, M.J.; Fu, Y.; Okita, T.W.; Barry, G.F.; Preiss, J.
    Adenosine 5'-diphosphate-glucose pyrophosphorylase from potato tuber. Significance of the N terminus of the small subunit for catalytic properties and heat stability (1995), Plant Physiol., 109, 245-251.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate the homotetrameric small subunit has much lower affinity for activator than the heterotetrameric enzyme Solanum tuberosum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 The genes of both subunits of enzyme are cloned, expression in Escherichia coli strain AC70R1-504, the one cloned small subunit lacks 10 amino acids of the N-terminus, and is defined as the truncated small subunit. A new cDNA clone of the small subunit encodes a polypeptide that has 10 amino acids more at the N-terminus than the truncated small subunits Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 phosphate
-
 Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.04
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, recombinant enzyme Solanum tuberosum
2.7.7.27 0.14
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 50000
-
 4 * 50000, the small subunit alone, SDS-PAGE Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 the recombinant enzyme containing the large subunit and the truncated small subunit, the recombinant enzyme containing the small subunit and the large subunit and the small subunit alone Solanum tuberosum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.27 ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-alpha-D-glucose the small and large subunits of the enzyme define the catalytic and regulatory subunits Solanum tuberosum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 additional information
-
 the small subunit alone is highly active but the large subunit alone has negligible catalytic activity Solanum tuberosum
2.7.7.27 20
-
 final preparation of the small subunits Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + ADP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.27 heterotetramer the large subunit is 51000 Da, major function is to modulate the regulatory properties of the enzyme and the small subunit is 50000 Da, major function is catalysis, SDS-PAGE Solanum tuberosum
2.7.7.27 homotetramer 4 * 50000, the small subunit alone, SDS-PAGE Solanum tuberosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.27 60
-
 large and truncated small subunits: 24% remaining activity, large and small subunits: 89% remaining activity, the small subunit: 98% remainig activity after heat treatment Solanum tuberosum
2.7.7.27 65
-
 large and truncated small subunits: 1.4% remaining activity, large and small subunits: 83% remaining activity, the small subunit: 102% remainig activity after heat treatment Solanum tuberosum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.04
-
 phosphate pH 8.0, 37°C, recombinant heterotetrameric enzyme, in absence of 3-phosphoglycerate Solanum tuberosum
2.7.7.27 0.08
-
 phosphate pH 8.0, 37°C, the small subunit of enzyme, in presence of 3 mM 3-phosphoglycerate Solanum tuberosum
2.7.7.27 0.63
-
 phosphate pH 8.0, 37°C, recombinant heterotetrameric enzyme, in presence of 3 mM 3-phosphoglycerate Solanum tuberosum