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Literature summary extracted from

  • Lee, Y.M.; Mukherjee, S.; Preiss, J.
    Covalent modification of Escherichia coli ADPglucose synthetase with 8-azido substrate analogs (1986), Arch. Biochem. Biophys., 244, 585-595.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 1,6-Hexanediol bisphosphate
-
Escherichia coli
2.7.7.27 D-fructose 1,6-bisphosphate
-
Escherichia coli

General Stability

EC Number General Stability Organism
2.7.7.27 the irradiation of enzyme in the presence of 8-N3ATP, fructose 1,6-phosphate and Mg2+ result in the covalent modification accompanying the loss of the enzyme activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 8-azaADP-glucose photoinactivation, competitive inhibition, ADP-glucose and ATP protects Escherichia coli
2.7.7.27 8-azaATP photoinactivation, competitive inhibition, 0.5 mol/mol enzyme subunit for complete inactivation, ADP-glucose and ATP protects Escherichia coli
2.7.7.27 8-N3ADPglucose
-
Escherichia coli
2.7.7.27 8-N3ATP
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.76
-
8-azaATP pH 8.5, 37°C, influence of activators on Km Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Escherichia coli
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.7.27 side-chain modification covalent modification with 8-azaATP and 8-azaADP-glucose accompanies irreversible loss of the enzyme catalytic activity, in the presence of light Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 8-azaATP + alpha-D-glucose 1-phosphate the photoaffinity labeling agent is used as a site specific probe of enzyme Escherichia coli diphosphate + 8-azaADP-glucose reverse reaction is biphasic r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
Escherichia coli diphosphate + ADP-glucose
-
?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.25
-
8-N3ADPglucose pH 8.5, 37°C Escherichia coli
2.7.7.27 1.25
-
8-N3ATP pH 8.5, 37°C Escherichia coli