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Literature summary extracted from

  • Preiss, J.; Crawford, K.; Downey, J.; Lammel, C.; Greenberg, E.
    Kinetic properties of Serratia marcescens adenosine 5-diphosphate glucose pyrophosphorylase (1976), J. Bacteriol., 127, 193-203.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 2-deoxy-D-ribose 5-phosphate no activation Serratia marcescens
2.7.7.27 2-keto-3-deoxy phosphogluconate activation Serratia marcescens
2.7.7.27 2-keto-3-deoxy phosphogluconate activation Serratia liquefaciens
2.7.7.27 3-phosphoglycerate activation Serratia marcescens
2.7.7.27 3-phosphoglycerate activation Serratia liquefaciens
2.7.7.27 3-phosphoglycerate slight activation Serratia marcescens
2.7.7.27 D-fructose 1,6-bisphosphate
-
 Serratia liquefaciens
2.7.7.27 D-fructose 1,6-bisphosphate slight activation Serratia marcescens
2.7.7.27 D-fructose 6-phosphate activation Serratia marcescens
2.7.7.27 D-fructose 6-phosphate activation Serratia liquefaciens
2.7.7.27 D-fructose 6-phosphate slight activation Serratia marcescens
2.7.7.27 D-Glucose 1,6-bisphosphate no activation Serratia marcescens
2.7.7.27 D-glucose 6-phosphate no activation Serratia marcescens
2.7.7.27 D-ribose 5-phosphate
-
 Serratia liquefaciens
2.7.7.27 D-ribose 5-phosphate activation Serratia marcescens
2.7.7.27 dihydroxyacetone phosphate no activation Serratia marcescens
2.7.7.27 dihydroxyacetone phosphate no activation Serratia liquefaciens
2.7.7.27 glyceraldehyde 3-phosphate no activation Serratia marcescens
2.7.7.27 additional information
-
 Serratia liquefaciens
2.7.7.27 additional information not: oxaloacetate, L-malate Serratia marcescens
2.7.7.27 additional information not: 2-oxoglutarate Serratia marcescens
2.7.7.27 additional information not: NADH, fumarate, succinate, acetyl-CoA, Glu, Ala, Asp, riboflavin 5'-phosphate Serratia marcescens
2.7.7.27 NADP+ no activation Serratia marcescens
2.7.7.27 phosphoenolpyruvate activation Serratia marcescens
2.7.7.27 phosphoenolpyruvate activation Serratia liquefaciens
2.7.7.27 phosphoenolpyruvate kinetics Serratia marcescens
2.7.7.27 pyridoxal 5'-phosphate activation Serratia marcescens
2.7.7.27 pyridoxal 5'-phosphate activation Serratia liquefaciens
2.7.7.27 pyridoxal 5'-phosphate slight activation Serratia marcescens
2.7.7.27 pyruvate not Serratia marcescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 ADP
-
 Serratia liquefaciens
2.7.7.27 ADP less effective than AMP; phosphoenolpyruvate, fructose bisphosphate, pyridoxal phosphate, NADPH, 3-phosphoglycerate, fructose 6-phosphate or pyruvate does not reverse Serratia marcescens
2.7.7.27 AMP
-
 Serratia liquefaciens
2.7.7.27 AMP ATCC 15365: very strong, ATP reverses Serratia marcescens
2.7.7.27 citrate
-
 Serratia marcescens
2.7.7.27 FAD
-
 Serratia marcescens
2.7.7.27 isocitrate
-
 Serratia marcescens
2.7.7.27 NAD+
-
 Serratia marcescens
2.7.7.27 phosphate
-
 Serratia liquefaciens
2.7.7.27 phosphate strain ATCC274 up to 10 mM Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 additional information
-
 additional information kinetic parameters Serratia marcescens
2.7.7.27 additional information
-
 additional information kinetic study Serratia marcescens
2.7.7.27 additional information
-
 additional information kinetic study Serratia liquefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+ requirement Serratia marcescens
2.7.7.27 Mg2+ requirement Serratia liquefaciens
2.7.7.27 Mg2+ ADPglucose synthesis Serratia marcescens
2.7.7.27 Mg2+ ADPglucose synthesis Serratia liquefaciens

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Serratia liquefaciens
-
-
-
2.7.7.27 Serratia marcescens
-
 ATCC 274, ATCC 15365 and wild-type strain HY
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 partial Serratia marcescens
2.7.7.27 strains ATCC 274 and ATCC 15365 Serratia marcescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 additional information distribution in genus Serratia Serratia marcescens
-
2.7.7.27 additional information distribution in genus Serratia Serratia liquefaciens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 0.125
-
 strain ATCC 15365 Serratia marcescens
2.7.7.27 0.287
-
 strain ATCC 274 Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate specific for ATP Serratia marcescens diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate specific for ATP Serratia liquefaciens diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate not: ITP Serratia marcescens diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate no substrate are GTP, dATP, CTP Serratia marcescens diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate not: dTTP, XTP Serratia marcescens diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate ADP-glucose synthesis at 50%, strain 274 or 75%, strain 15365 the rate of pyrophosphorolysis Serratia marcescens diphosphate + ADP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.27 More enzyme exist as tetrameric and dimeric forms Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.27 7.5
-
 Hepes buffer Serratia marcescens
2.7.7.27 8
-
 Hepes buffer Serratia marcescens
2.7.7.27 8.5
-
 Tris buffer Serratia marcescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.015
-
 AMP pH 8.0, 37°C, strain ATCC 15365 Serratia marcescens
2.7.7.27 0.031
-
 AMP pH 8.0, 37°C, strain ATCC 274 Serratia marcescens
2.7.7.27 0.235
-
 ADP pH 8.0, 37°C, strain ATCC 15365 Serratia marcescens
2.7.7.27 8.7
-
 phosphate pH 8.0, 37°C, strain ATCC 15365 Serratia marcescens