BRENDA - Enzyme Database

Biosynthesis of bacterial glycogen: purification and properties of Salmonella typhimurium LT-2 adenosine diphosphate glucose pyrophosphorylase

Lehmann, M.; Preiss, J.; J. Bacteriol. 143, 120-127 (1980)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.27
2,3-diphosphoglycerate
activation; allosteric activator
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
2-phospho-D-glycerate
activation; slight activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
3-phosphoglycerate
no activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
6-phosphogluconate
slight activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
D-fructose 1,6-bisphosphate
allosteric activator; physiological modulator
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
D-Glucose 1,6-bisphosphate
slight activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
D-ribose 5-phosphate
no activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
glyceraldehyde 3-phosphate
slight activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
NADP+
slight activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
NADPH
activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
pyridoxal 5'-phosphate
activation; allosteric activator
Salmonella enterica subsp. enterica serovar Typhimurium
General Stability
EC Number
General Stability
Organism
2.7.7.27
glycerol, 20% stabilizes
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.27
ADP
allosteric inhibitor
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
AMP
allosteric inhibitor
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.27
2
-
ATP
pH 8.0, 37C, strain JP102, kinetic study
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
2.4
-
ATP
pH 8.0, 37C, strain LT-2, kinetic study
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.27
Mg2+
requirement
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.27
additional information
-
amino acid composition and comparison
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
48000
-
4 * 48000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
190000
-
sucrose density gradient centrifugation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
195000
-
PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.27
Salmonella enterica subsp. enterica serovar Typhimurium
-
LT-2 mutant JP102; strains LT-2
-
2.7.7.27
Salmonella enterica subsp. enterica serovar Typhimurium LT2
-
strains LT-2
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.27
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.7.27
106
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability
EC Number
Storage Stability
Organism
2.7.7.27
-70C, in 50 mM Tris-HCl buffer, pH 7.2, 10 mM potassium phosphate, 0.5 mM dithioerythritol, 20% glycerol, stable
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643134
Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate + ADP-glucose
-
643134
Salmonella enterica subsp. enterica serovar Typhimurium
r
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643134
Salmonella enterica subsp. enterica serovar Typhimurium LT2
diphosphate + ADP-glucose
-
643134
Salmonella enterica subsp. enterica serovar Typhimurium LT2
r
Subunits
EC Number
Subunits
Commentary
Organism
2.7.7.27
homotetramer
4 * 48000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.27
0.028
-
AMP
pH 8.0, 37C, strain LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
0.032
-
AMP
pH 8.0, 37C, strain JP102, in presence of 0.25 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
0.096
-
AMP
pH 8.0, 37C, strain JP102, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
0.11
-
AMP
pH 8.0, 37C, strain LT-2, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
0.42
-
ADP
pH 8.0, 37C, strains JP102 and LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
0.93
-
phosphate
pH 8.0, 37C, strains LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
1
-
ADP
pH 8.0, 37C, strains JP102, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
1.1
-
ADP
pH 8.0, 37C, strains LT-2, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
1.3
-
phosphate
pH 8.0, 37C, strains JP102, in presence of 0.25 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
1.7
-
phosphate
pH 8.0, 37C, strains LT-2, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
2
-
phosphate
pH 8.0, 37C, strains JP102, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.27
2,3-diphosphoglycerate
activation; allosteric activator
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
2-phospho-D-glycerate
activation; slight activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
3-phosphoglycerate
no activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
6-phosphogluconate
slight activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
D-fructose 1,6-bisphosphate
allosteric activator; physiological modulator
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
D-Glucose 1,6-bisphosphate
slight activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
D-ribose 5-phosphate
no activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
glyceraldehyde 3-phosphate
slight activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
NADP+
slight activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
NADPH
activation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
pyridoxal 5'-phosphate
activation; allosteric activator
Salmonella enterica subsp. enterica serovar Typhimurium
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.27
glycerol, 20% stabilizes
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.27
ADP
allosteric inhibitor
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
AMP
allosteric inhibitor
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.27
0.028
-
AMP
pH 8.0, 37C, strain LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
0.032
-
AMP
pH 8.0, 37C, strain JP102, in presence of 0.25 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
0.096
-
AMP
pH 8.0, 37C, strain JP102, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
0.11
-
AMP
pH 8.0, 37C, strain LT-2, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
0.42
-
ADP
pH 8.0, 37C, strains JP102 and LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
0.93
-
phosphate
pH 8.0, 37C, strains LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
1
-
ADP
pH 8.0, 37C, strains JP102, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
1.1
-
ADP
pH 8.0, 37C, strains LT-2, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
1.3
-
phosphate
pH 8.0, 37C, strains JP102, in presence of 0.25 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
1.7
-
phosphate
pH 8.0, 37C, strains LT-2, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
2
-
phosphate
pH 8.0, 37C, strains JP102, in presence of 1 mM fructose 1,6-bisphosphate
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.27
2
-
ATP
pH 8.0, 37C, strain JP102, kinetic study
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
2.4
-
ATP
pH 8.0, 37C, strain LT-2, kinetic study
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.27
Mg2+
requirement
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.27
additional information
-
amino acid composition and comparison
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
48000
-
4 * 48000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
190000
-
sucrose density gradient centrifugation
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27
195000
-
PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.27
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.7.27
106
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.7.27
-70C, in 50 mM Tris-HCl buffer, pH 7.2, 10 mM potassium phosphate, 0.5 mM dithioerythritol, 20% glycerol, stable
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643134
Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate + ADP-glucose
-
643134
Salmonella enterica subsp. enterica serovar Typhimurium
r
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643134
Salmonella enterica subsp. enterica serovar Typhimurium LT2
diphosphate + ADP-glucose
-
643134
Salmonella enterica subsp. enterica serovar Typhimurium LT2
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.7.27
homotetramer
4 * 48000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium