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Literature summary extracted from

  • Yung, S.G.; Preiss, J.
    Biosynthesis of bacterial glycogen: purification and structural properties of Rhodospirillum tenue adenosine diphosphate glucose synthetase (1981), J. Bacteriol., 147, 101-109.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.7.27 no reducing reagent required during purification Rhodocyclus tenuis
2.7.7.27 phosphate stabilizes Rhodocyclus tenuis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+ requirement Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 Mg2+ requirement Escherichia coli
2.7.7.27 Mg2+ requirement Cereibacter sphaeroides
2.7.7.27 Mg2+ requirement Spinacia oleracea
2.7.7.27 Mg2+ requirement Rubrivivax gelatinosus
2.7.7.27 Mg2+ requirement Rhodopseudomonas palustris
2.7.7.27 Mg2+ requirement Rhodobacter capsulatus
2.7.7.27 Mg2+ requirement Rhodospirillum rubrum
2.7.7.27 Mg2+ requirement Rhodomicrobium vannielii
2.7.7.27 Mg2+ requirement Magnetospirillum molischianum
2.7.7.27 Mg2+ requirement Blastochloris viridis
2.7.7.27 Mg2+ requirement Rhodoblastus acidophilus
2.7.7.27 Mg2+ requirement Rhodopila globiformis
2.7.7.27 Mg2+ requirement Rhodocyclus tenuis
2.7.7.27 Mg2+ requirement Magnetospirillum fulvum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 additional information
-
-
 Spinacia oleracea
2.7.7.27 additional information
-
-
 Rubrivivax gelatinosus
2.7.7.27 additional information
-
-
 Rhodopseudomonas palustris
2.7.7.27 additional information
-
-
 Rhodobacter capsulatus
2.7.7.27 additional information
-
-
 Rhodomicrobium vannielii
2.7.7.27 additional information
-
-
 Magnetospirillum molischianum
2.7.7.27 additional information
-
-
 Blastochloris viridis
2.7.7.27 additional information
-
-
 Rhodoblastus acidophilus
2.7.7.27 additional information
-
-
 Rhodopila globiformis
2.7.7.27 additional information
-
-
 Magnetospirillum fulvum
2.7.7.27 additional information
-
 N-terminal sequence and comparison Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 additional information
-
 N-terminal sequence and comparison Escherichia coli
2.7.7.27 additional information
-
 N-terminal sequence and comparison Cereibacter sphaeroides
2.7.7.27 additional information
-
 N-terminal sequence and comparison Rhodospirillum rubrum
2.7.7.27 additional information
-
 N-terminal sequence and comparison Rhodocyclus tenuis
2.7.7.27 additional information
-
 amino acid composition and comparison Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 additional information
-
 amino acid composition and comparison Escherichia coli
2.7.7.27 additional information
-
 amino acid composition and comparison Cereibacter sphaeroides
2.7.7.27 additional information
-
 amino acid composition and comparison Rhodospirillum rubrum
2.7.7.27 additional information
-
 amino acid composition and comparison Rhodocyclus tenuis
2.7.7.27 51000
-
 4 * 51000, SDS-PAGE Escherichia coli
2.7.7.27 51000
-
 4 * 51000, SDS-PAGE Rhodocyclus tenuis
2.7.7.27 215000
-
 sucrose density gradient centrifugation Rhodocyclus tenuis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Blastochloris viridis
-
-
-
2.7.7.27 Cereibacter sphaeroides
-
 strains 3244 and JT
-
2.7.7.27 Escherichia coli
-
-
-
2.7.7.27 Magnetospirillum fulvum
-
-
-
2.7.7.27 Magnetospirillum molischianum
-
-
-
2.7.7.27 Rhodobacter capsulatus
-
-
-
2.7.7.27 Rhodoblastus acidophilus
-
-
-
2.7.7.27 Rhodocyclus tenuis
-
-
-
2.7.7.27 Rhodomicrobium vannielii
-
-
-
2.7.7.27 Rhodopila globiformis
-
-
-
2.7.7.27 Rhodopseudomonas palustris
-
-
-
2.7.7.27 Rhodospirillum rubrum
-
-
-
2.7.7.27 Rubrivivax gelatinosus
-
-
-
2.7.7.27 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.7.7.27 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27
-
 Rhodocyclus tenuis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 114
-
-
 Rhodocyclus tenuis

Storage Stability

EC Number Storage Stability Organism
2.7.7.27 0°C, in 30 mM potassium phosphate buffer, pH 7, 4 days Rhodocyclus tenuis
2.7.7.27 4°C, 30-50% loss of activity overnight Rhodocyclus tenuis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Escherichia coli diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Cereibacter sphaeroides diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Spinacia oleracea diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Rubrivivax gelatinosus diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Rhodopseudomonas palustris diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Rhodobacter capsulatus diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Rhodospirillum rubrum diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Rhodomicrobium vannielii diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Magnetospirillum molischianum diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Blastochloris viridis diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Rhodoblastus acidophilus diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Rhodopila globiformis diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Rhodocyclus tenuis diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Magnetospirillum fulvum diphosphate + ADP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.27 homotetramer
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 homotetramer
-
 Cereibacter sphaeroides
2.7.7.27 homotetramer
-
 Spinacia oleracea
2.7.7.27 homotetramer
-
 Rhodospirillum rubrum
2.7.7.27 homotetramer 4 * 51000, SDS-PAGE Escherichia coli
2.7.7.27 homotetramer 4 * 51000, SDS-PAGE Rhodocyclus tenuis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.27 25
-
 4 days, stable in the presence of 30 mM potassium phosphate, pH 7 Rhodocyclus tenuis
2.7.7.27 37
-
 4 days, stable in the presence of 30 mM potassium phosphate, pH 7 Rhodocyclus tenuis