BRENDA - Enzyme Database

Regulatiory and structural properties of the cyanobacterial ADPglucose pyrophophorylases

Iglesias, A.A.; Kakefuda, G.; Preiss, J.; Plant Physiol. 97, 1187-1195 (1991)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.27
2,3-diphosphoglycerate
no activation
Anabaena sp.
2.7.7.27
2,3-diphosphoglycerate
no activation
Synechocystis sp.
2.7.7.27
2-phospho-D-glycerate
activation; no activation
Anabaena sp.
2.7.7.27
2-phospho-D-glycerate
activation; no activation
Synechocystis sp.
2.7.7.27
3-phosphoglycerate
allosteric activation; kinetics
Anabaena sp.
2.7.7.27
3-phosphoglycerate
allosteric activation; kinetics
Synechocystis sp.
2.7.7.27
ADP
no activation
Anabaena sp.
2.7.7.27
ADP
no activation
Synechocystis sp.
2.7.7.27
AMP
no activation
Anabaena sp.
2.7.7.27
AMP
no activation
Synechocystis sp.
2.7.7.27
D-fructose 1,6-bisphosphate
slight activation
Anabaena sp.
2.7.7.27
D-fructose 1,6-bisphosphate
slight activation
Synechocystis sp.
2.7.7.27
D-fructose 6-phosphate
activation; slight activation
Anabaena sp.
2.7.7.27
D-fructose 6-phosphate
activation; slight activation
Synechocystis sp.
2.7.7.27
D-Glucose 1,6-bisphosphate
no activation
Anabaena sp.
2.7.7.27
D-Glucose 1,6-bisphosphate
no activation
Synechocystis sp.
2.7.7.27
dihydroxyacetone phosphate
no activation
Anabaena sp.
2.7.7.27
dihydroxyacetone phosphate
no activation
Synechocystis sp.
2.7.7.27
glyceraldehyde 3-phosphate
slight activation
Anabaena sp.
2.7.7.27
glyceraldehyde 3-phosphate
slight activation
Synechocystis sp.
2.7.7.27
additional information
-
Anabaena sp.
2.7.7.27
additional information
-
Synechocystis sp.
2.7.7.27
phosphoenolpyruvate
activation; slight activation
Anabaena sp.
2.7.7.27
phosphoenolpyruvate
activation; slight activation
Synechocystis sp.
2.7.7.27
pyridoxal 5'-phosphate
activation
Anabaena sp.
2.7.7.27
pyridoxal 5'-phosphate
activation
Synechocystis sp.
2.7.7.27
pyruvate
not
Anabaena sp.
2.7.7.27
pyruvate
not
Synechocystis sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.27
ADP
not
Anabaena sp.
2.7.7.27
ADP
not
Synechocystis sp.
2.7.7.27
AMP
not
Anabaena sp.
2.7.7.27
AMP
not
Synechocystis sp.
2.7.7.27
Phenylglyoxal
in the presence of 3-phosphoglycerate
Anabaena sp.
2.7.7.27
Phenylglyoxal
in the presence of 3-phosphoglycerate
Synechocystis sp.
2.7.7.27
phosphate
-
Anabaena sp.
2.7.7.27
phosphate
-
Synechocystis sp.
2.7.7.27
Rabbit antiserum raised against spinach enzyme
not antiserum against E. coli enzyme
Anabaena sp.
2.7.7.27
Rabbit antiserum raised against spinach enzyme
not antiserum against E. coli enzyme
Synechocystis sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.27
0.13
-
alpha-D-glucose 1-phosphate
pH 7.0, 37°C, kinetic study
Anabaena sp.
2.7.7.27
0.18
-
alpha-D-glucose 1-phosphate
pH 7.0, 37°C, kinetic study
Synechocystis sp.
2.7.7.27
1.55
-
ATP
pH 7.0, 37°C, kinetic study
Anabaena sp.
2.7.7.27
3.2
-
ATP
pH 7.0, 37°C, kinetic study
Synechocystis sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.27
Mg2+
requirement
Synechocystis sp.
2.7.7.27
Mg2+
requirement
Anabaena sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.27
53000
-
4 * 53000, SDS-PAGE
Synechocystis sp.
2.7.7.27
216000
-
gel filtration
Synechocystis sp.
2.7.7.27
225000
-
gel filtration
Anabaena sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.27
Anabaena sp.
-
PCC 7120, cyanobacterium
-
2.7.7.27
Synechocystis sp.
-
PCC 6803, cyanobacterium
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.27
to near homogeneity
Anabaena sp.
2.7.7.27
to near homogeneity
Synechocystis sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.27
111
-
-
Anabaena sp.
2.7.7.27
134
-
-
Synechocystis sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643123
Anabaena sp.
diphosphate + ADP-glucose
-
-
-
-
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643123
Synechocystis sp.
diphosphate + ADP-glucose
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.7.27
homotetramer
4 * 53000, SDS-PAGE
Synechocystis sp.
2.7.7.27
homotetramer
-
Anabaena sp.
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.27
0.044
-
phosphate
pH 7.0, 37°C, in absence of 3-phosphoglycerate
Anabaena sp.
2.7.7.27
0.095
-
phosphate
pH 7.0, 37°C, in absence of 3-phosphoglycerate
Synechocystis sp.
2.7.7.27
0.46
-
phosphate
pH 7.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate
Anabaena sp.
2.7.7.27
0.57
-
phosphate
pH 7.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate
Synechocystis sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.27
2,3-diphosphoglycerate
no activation
Anabaena sp.
2.7.7.27
2,3-diphosphoglycerate
no activation
Synechocystis sp.
2.7.7.27
2-phospho-D-glycerate
activation; no activation
Anabaena sp.
2.7.7.27
2-phospho-D-glycerate
activation; no activation
Synechocystis sp.
2.7.7.27
3-phosphoglycerate
allosteric activation; kinetics
Anabaena sp.
2.7.7.27
3-phosphoglycerate
allosteric activation; kinetics
Synechocystis sp.
2.7.7.27
ADP
no activation
Anabaena sp.
2.7.7.27
ADP
no activation
Synechocystis sp.
2.7.7.27
AMP
no activation
Anabaena sp.
2.7.7.27
AMP
no activation
Synechocystis sp.
2.7.7.27
D-fructose 1,6-bisphosphate
slight activation
Anabaena sp.
2.7.7.27
D-fructose 1,6-bisphosphate
slight activation
Synechocystis sp.
2.7.7.27
D-fructose 6-phosphate
activation; slight activation
Anabaena sp.
2.7.7.27
D-fructose 6-phosphate
activation; slight activation
Synechocystis sp.
2.7.7.27
D-Glucose 1,6-bisphosphate
no activation
Anabaena sp.
2.7.7.27
D-Glucose 1,6-bisphosphate
no activation
Synechocystis sp.
2.7.7.27
dihydroxyacetone phosphate
no activation
Anabaena sp.
2.7.7.27
dihydroxyacetone phosphate
no activation
Synechocystis sp.
2.7.7.27
glyceraldehyde 3-phosphate
slight activation
Anabaena sp.
2.7.7.27
glyceraldehyde 3-phosphate
slight activation
Synechocystis sp.
2.7.7.27
additional information
-
Anabaena sp.
2.7.7.27
additional information
-
Synechocystis sp.
2.7.7.27
phosphoenolpyruvate
activation; slight activation
Anabaena sp.
2.7.7.27
phosphoenolpyruvate
activation; slight activation
Synechocystis sp.
2.7.7.27
pyridoxal 5'-phosphate
activation
Anabaena sp.
2.7.7.27
pyridoxal 5'-phosphate
activation
Synechocystis sp.
2.7.7.27
pyruvate
not
Anabaena sp.
2.7.7.27
pyruvate
not
Synechocystis sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.27
ADP
not
Anabaena sp.
2.7.7.27
ADP
not
Synechocystis sp.
2.7.7.27
AMP
not
Anabaena sp.
2.7.7.27
AMP
not
Synechocystis sp.
2.7.7.27
Phenylglyoxal
in the presence of 3-phosphoglycerate
Anabaena sp.
2.7.7.27
Phenylglyoxal
in the presence of 3-phosphoglycerate
Synechocystis sp.
2.7.7.27
phosphate
-
Anabaena sp.
2.7.7.27
phosphate
-
Synechocystis sp.
2.7.7.27
Rabbit antiserum raised against spinach enzyme
not antiserum against E. coli enzyme
Anabaena sp.
2.7.7.27
Rabbit antiserum raised against spinach enzyme
not antiserum against E. coli enzyme
Synechocystis sp.
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.27
0.044
-
phosphate
pH 7.0, 37°C, in absence of 3-phosphoglycerate
Anabaena sp.
2.7.7.27
0.095
-
phosphate
pH 7.0, 37°C, in absence of 3-phosphoglycerate
Synechocystis sp.
2.7.7.27
0.46
-
phosphate
pH 7.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate
Anabaena sp.
2.7.7.27
0.57
-
phosphate
pH 7.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate
Synechocystis sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.27
0.13
-
alpha-D-glucose 1-phosphate
pH 7.0, 37°C, kinetic study
Anabaena sp.
2.7.7.27
0.18
-
alpha-D-glucose 1-phosphate
pH 7.0, 37°C, kinetic study
Synechocystis sp.
2.7.7.27
1.55
-
ATP
pH 7.0, 37°C, kinetic study
Anabaena sp.
2.7.7.27
3.2
-
ATP
pH 7.0, 37°C, kinetic study
Synechocystis sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.27
Mg2+
requirement
Synechocystis sp.
2.7.7.27
Mg2+
requirement
Anabaena sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.27
53000
-
4 * 53000, SDS-PAGE
Synechocystis sp.
2.7.7.27
216000
-
gel filtration
Synechocystis sp.
2.7.7.27
225000
-
gel filtration
Anabaena sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.27
to near homogeneity
Anabaena sp.
2.7.7.27
to near homogeneity
Synechocystis sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.27
111
-
-
Anabaena sp.
2.7.7.27
134
-
-
Synechocystis sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643123
Anabaena sp.
diphosphate + ADP-glucose
-
-
-
-
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643123
Synechocystis sp.
diphosphate + ADP-glucose
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.7.27
homotetramer
4 * 53000, SDS-PAGE
Synechocystis sp.
2.7.7.27
homotetramer
-
Anabaena sp.