BRENDA - Enzyme Database

Purification and properties of nonproteolytic degraded ADPglucose pyrophosphorylase from maize endosperm

Plaxton, W.C.; Preiss, J.; Plant Physiol. 83, 105-112 (1987)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.27
2,3-diphosphoglycerate
no activation
Zea mays
2.7.7.27
2-phospho-D-glycerate
no activation
Zea mays
2.7.7.27
3-phosphoglycerate
activation; allosteric activation
Zea mays
2.7.7.27
D-fructose 1,6-bisphosphate
no activation
Zea mays
2.7.7.27
D-fructose 6-phosphate
activation; less effective than 3-phosphoglycerate
Zea mays
2.7.7.27
D-Glucose 1,6-bisphosphate
no activation
Zea mays
2.7.7.27
D-glucose 6-phosphate
activation; less effective than 3-phosphoglycerate
Zea mays
2.7.7.27
dihydroxyacetone phosphate
activation, less effective than 3-phosphoglycerate
Zea mays
2.7.7.27
glyceraldehyde 3-phosphate
no activation
Zea mays
2.7.7.27
additional information
not: fructose 2,6-bisphosphate, Ca2+/calmodulin
Zea mays
2.7.7.27
phosphoenolpyruvate
activation; less effective than 3-phosphoglycerate
Zea mays
2.7.7.27
pyruvate
not
Zea mays
General Stability
EC Number
General Stability
Organism
2.7.7.27
PMSF and chymostatin prevent proteolysis during purification, not p-aminobenzamidine, N-alpha-p-tosyl-L-lysine chloromethylketone, NEM, PCMB, benzamidine, leupeptin, pepstatin or EDTA
Zea mays
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.27
F-
NaF
Zea mays
2.7.7.27
phosphate
allosteric inhibitor
Zea mays
2.7.7.27
Rabbit antiserum raised against spinach enzyme
-
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.27
additional information
-
additional information
comparison of Km of proteolyzed and non-proteolyzed enzyme at pH 7.4 and pH 6.8; kinetic study
Zea mays
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.27
54000
-
4 * 54000 SDS-PAGE
Zea mays
2.7.7.27
230000
-
gel filtration
Zea mays
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.27
Zea mays
-
starchy maize
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.27
endosperm; partial; whole kernels
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.27
endosperm
-
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.7.27
34
-
-
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643120
Zea mays
diphosphate + ADP-glucose
-
643120
Zea mays
r
Subunits
EC Number
Subunits
Commentary
Organism
2.7.7.27
homotetramer
4 * 54000 SDS-PAGE
Zea mays
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.27
7.4
-
-
Zea mays
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.27
0.44
-
phosphate
pH 7.4, 37░C, in presence of 1 mM 3-phosphoglycerate
Zea mays
2.7.7.27
9.8
-
phosphate
pH 7.4, 37░C, in presence of 10 mM 3-phosphoglycerate
Zea mays
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.27
2,3-diphosphoglycerate
no activation
Zea mays
2.7.7.27
2-phospho-D-glycerate
no activation
Zea mays
2.7.7.27
3-phosphoglycerate
activation; allosteric activation
Zea mays
2.7.7.27
D-fructose 1,6-bisphosphate
no activation
Zea mays
2.7.7.27
D-fructose 6-phosphate
activation; less effective than 3-phosphoglycerate
Zea mays
2.7.7.27
D-Glucose 1,6-bisphosphate
no activation
Zea mays
2.7.7.27
D-glucose 6-phosphate
activation; less effective than 3-phosphoglycerate
Zea mays
2.7.7.27
dihydroxyacetone phosphate
activation, less effective than 3-phosphoglycerate
Zea mays
2.7.7.27
glyceraldehyde 3-phosphate
no activation
Zea mays
2.7.7.27
additional information
not: fructose 2,6-bisphosphate, Ca2+/calmodulin
Zea mays
2.7.7.27
phosphoenolpyruvate
activation; less effective than 3-phosphoglycerate
Zea mays
2.7.7.27
pyruvate
not
Zea mays
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.27
PMSF and chymostatin prevent proteolysis during purification, not p-aminobenzamidine, N-alpha-p-tosyl-L-lysine chloromethylketone, NEM, PCMB, benzamidine, leupeptin, pepstatin or EDTA
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.27
F-
NaF
Zea mays
2.7.7.27
phosphate
allosteric inhibitor
Zea mays
2.7.7.27
Rabbit antiserum raised against spinach enzyme
-
Zea mays
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.27
0.44
-
phosphate
pH 7.4, 37░C, in presence of 1 mM 3-phosphoglycerate
Zea mays
2.7.7.27
9.8
-
phosphate
pH 7.4, 37░C, in presence of 10 mM 3-phosphoglycerate
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.27
additional information
-
additional information
comparison of Km of proteolyzed and non-proteolyzed enzyme at pH 7.4 and pH 6.8; kinetic study
Zea mays
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.27
54000
-
4 * 54000 SDS-PAGE
Zea mays
2.7.7.27
230000
-
gel filtration
Zea mays
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.27
endosperm; partial; whole kernels
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.27
endosperm
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.7.27
34
-
-
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643120
Zea mays
diphosphate + ADP-glucose
-
643120
Zea mays
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.7.27
homotetramer
4 * 54000 SDS-PAGE
Zea mays
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.27
7.4
-
-
Zea mays