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Literature summary extracted from

  • Plaxton, W.C.; Preiss, J.
    Purification and properties of nonproteolytic degraded ADPglucose pyrophosphorylase from maize endosperm (1987), Plant Physiol., 83, 105-112.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 2,3-diphosphoglycerate no activation Zea mays
2.7.7.27 2-phospho-D-glycerate no activation Zea mays
2.7.7.27 3-phosphoglycerate allosteric activation Zea mays
2.7.7.27 3-phosphoglycerate activation Zea mays
2.7.7.27 D-fructose 1,6-bisphosphate no activation Zea mays
2.7.7.27 D-fructose 6-phosphate activation Zea mays
2.7.7.27 D-fructose 6-phosphate less effective than 3-phosphoglycerate Zea mays
2.7.7.27 D-Glucose 1,6-bisphosphate no activation Zea mays
2.7.7.27 D-glucose 6-phosphate activation Zea mays
2.7.7.27 D-glucose 6-phosphate less effective than 3-phosphoglycerate Zea mays
2.7.7.27 dihydroxyacetone phosphate activation, less effective than 3-phosphoglycerate Zea mays
2.7.7.27 glyceraldehyde 3-phosphate no activation Zea mays
2.7.7.27 additional information not: fructose 2,6-bisphosphate, Ca2+/calmodulin Zea mays
2.7.7.27 phosphoenolpyruvate activation Zea mays
2.7.7.27 phosphoenolpyruvate less effective than 3-phosphoglycerate Zea mays
2.7.7.27 pyruvate not Zea mays

General Stability

EC Number General Stability Organism
2.7.7.27 PMSF and chymostatin prevent proteolysis during purification, not p-aminobenzamidine, N-alpha-p-tosyl-L-lysine chloromethylketone, NEM, PCMB, benzamidine, leupeptin, pepstatin or EDTA Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 F- NaF Zea mays
2.7.7.27 phosphate allosteric inhibitor Zea mays
2.7.7.27 Rabbit antiserum raised against spinach enzyme
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 additional information
-
 additional information kinetic study Zea mays
2.7.7.27 additional information
-
 additional information comparison of Km of proteolyzed and non-proteolyzed enzyme at pH 7.4 and pH 6.8 Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 54000
-
 4 * 54000 SDS-PAGE Zea mays
2.7.7.27 230000
-
 gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Zea mays
-
 starchy maize
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 endosperm Zea mays
2.7.7.27 partial Zea mays
2.7.7.27 whole kernels Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 endosperm
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 34
-
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Zea mays diphosphate + ADP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.27 homotetramer 4 * 54000 SDS-PAGE Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.27 7.4
-
-
 Zea mays

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.44
-
 phosphate pH 7.4, 37°C, in presence of 1 mM 3-phosphoglycerate Zea mays
2.7.7.27 9.8
-
 phosphate pH 7.4, 37°C, in presence of 10 mM 3-phosphoglycerate Zea mays