EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.27 | 3-phosphoglycerate | allosteric activation | Zea mays | |
2.7.7.27 | 3-phosphoglycerate | activation | Zea mays | |
2.7.7.27 | D-fructose 6-phosphate | activation | Zea mays | |
2.7.7.27 | D-fructose 6-phosphate | less effective than 3-phosphoglycerate | Zea mays | |
2.7.7.27 | D-ribose 5-phosphate | activation | Zea mays | |
2.7.7.27 | D-ribose 5-phosphate | less effective than 3-phosphoglycerate | Zea mays |
EC Number | General Stability | Organism |
---|---|---|
2.7.7.27 | freeze-thawing reduces activity by 70%, high ionic strength, ATP and Mg2+ stabilize | Zea mays |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.27 | ADP | - |
Zea mays | |
2.7.7.27 | AMP | - |
Zea mays | |
2.7.7.27 | Cl- | not at 5 mM, only at 10 mM | Zea mays | |
2.7.7.27 | diphosphate | - |
Zea mays | |
2.7.7.27 | F- | not at 5 mM, only at 10 mM | Zea mays | |
2.7.7.27 | NO3- | not at 5 mM, only at 10 mM | Zea mays | |
2.7.7.27 | phosphate | allosteric inhibitor | Zea mays | |
2.7.7.27 | phosphoenolpyruvate | - |
Zea mays | |
2.7.7.27 | pyruvate | - |
Zea mays | |
2.7.7.27 | SO42- | 5 mM, weak | Zea mays | |
2.7.7.27 | UDP-glucose | - |
Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.27 | 0.032 | - |
ATP | pH 7.4, 37°C | Zea mays | |
2.7.7.27 | 0.033 | - |
diphosphate | pH 7.4, 37°C | Zea mays | |
2.7.7.27 | 0.19 | - |
alpha-D-glucose 1-phosphate | pH 7.4, 37°C | Zea mays | |
2.7.7.27 | 0.62 | - |
ADP-glucose | pH 7.4, 37°C | Zea mays |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.27 | Mg2+ | requirement | Zea mays | |
2.7.7.27 | Mg2+ | 6-8 mM | Zea mays | |
2.7.7.27 | additional information | no activation by Mn2+, Zn2+, NH4+, Na+ | Zea mays |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.27 | ATP + alpha-D-glucose 1-phosphate | Zea mays | key regulatory enzyme of starch biosynthesis | diphosphate + ADP-glucose | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.27 | Zea mays | - |
sweet corn, var. Golden Beauty | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.27 | partial | Zea mays |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.27 | seed | - |
Zea mays | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.27 | 4.4 | - |
- |
Zea mays |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.7.27 | -20°C, in 60% (NH4)2SO4, 15 weeks | Zea mays |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.27 | ATP + alpha-D-glucose 1-phosphate | specific for ATP | Zea mays | diphosphate + ADP-glucose | - |
r | |
2.7.7.27 | ATP + alpha-D-glucose 1-phosphate | not: TTP | Zea mays | diphosphate + ADP-glucose | - |
r | |
2.7.7.27 | ATP + alpha-D-glucose 1-phosphate | no substrate is UTP | Zea mays | diphosphate + ADP-glucose | - |
r | |
2.7.7.27 | ATP + alpha-D-glucose 1-phosphate | key regulatory enzyme of starch biosynthesis | Zea mays | diphosphate + ADP-glucose | - |
r |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.27 | 7 | 7.5 | ADP-glucose synthesis, Tris buffer | Zea mays |
2.7.7.27 | 7 | 8 | ADP-glucose synthesis, HEPES buffer | Zea mays |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.27 | 6 | 8.3 | about half-maximal activity at pH 6.0 and 8.3, Tris buffer | Zea mays |
2.7.7.27 | 6.1 | 8.5 | about half-maximal activity at pH 6.1 and about 80% of maximal activity at pH 8.5, HEPES buffer | Zea mays |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.27 | 0.00038 | - |
diphosphate | pH 7.4, 37°C | Zea mays |