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Literature summary extracted from

  • Copeland, L.; Preiss, J.
    Purification of spinach leaf ADPglucose pyrophophorylase (1981), Plant Physiol., 68, 996-1001.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate allosteric activation Spinacia oleracea
2.7.7.27 3-phosphoglycerate activation Spinacia oleracea
2.7.7.27 D-fructose 1,6-bisphosphate activation of pyrophosphorolysis Spinacia oleracea
2.7.7.27 D-fructose 1,6-bisphosphate activation of ADP-glucose synthesis Spinacia oleracea
2.7.7.27 D-fructose 6-phosphate activation Spinacia oleracea
2.7.7.27 D-fructose 6-phosphate activation of ADPglucose synthesis Spinacia oleracea
2.7.7.27 phosphoenolpyruvate activation Spinacia oleracea
2.7.7.27 phosphoenolpyruvate activation of pyrophosphorolysis Spinacia oleracea
2.7.7.27 phosphoenolpyruvate ADPglucose synthesis Spinacia oleracea

General Stability

EC Number General Stability Organism
2.7.7.27 polyvinylpyrrolidone, i.e. PVP fractionation stabilizes during purification, 20% sucrose and 30 mM phosphate stabilize during purification Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 phosphate allosteric inhibitor Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.031
-
 alpha-D-glucose 1-phosphate pH 7.3, 37°C, in presence of 1 mM 3-phosphoglycerate Spinacia oleracea
2.7.7.27 0.045
-
 ATP pH 7.3, 37°C, in presence of 1 mM 3-phosphoglycerate Spinacia oleracea
2.7.7.27 0.105
-
 alpha-D-glucose 1-phosphate pH 7.3, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
2.7.7.27 0.32
-
 ATP pH 7.3, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.27 soluble
-
 Spinacia oleracea
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 44000
-
 x * 44000 + x * 48000, SDS-PAGE Spinacia oleracea
2.7.7.27 48000
-
 x * 44000 + x * 48000, SDS-PAGE Spinacia oleracea
2.7.7.27 206000
-
 sucrose density gradient ultracentrifugation Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Spinacia oleracea one of the main regulatory steps in starch biosynthesis in plants diphosphate + ADP-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 156
-
-
 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.7.7.27 4°C, in 50 mM HEPES buffer, pH 7, 20% sucrose, 0.2 mM dithioerythritol, 1 mM EDTA, 2 weeks, about 60% loss of activity within 1 month Spinacia oleracea
2.7.7.27 frozen or 4°C, most stable in buffer containing 20% sucrose and 30 mM phosphate Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Spinacia oleracea diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate one of the main regulatory steps in starch biosynthesis in plants Spinacia oleracea diphosphate + ADP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.27 heterotetramer x * 44000 + x * 48000, SDS-PAGE Spinacia oleracea
2.7.7.27 homotetramer
-
 Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.045
-
 phosphate pH 8.0, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
2.7.7.27 0.064
-
 phosphate pH 7.3, 37°C, in absence of phosphoglycerate Spinacia oleracea
2.7.7.27 0.97
-
 phosphate pH 7.3, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea
2.7.7.27 1.27
-
 phosphate pH 7.3, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea