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Literature summary extracted from

  • Ghosh, H.P.; Preiss, J.
    Adenosine diphosphate glucose pyrophosphorylase. A regulatory enzyme in the biosynthesis of starch in spinach leaf chloroplasts (1966), J. Biol. Chem., 241, 4491-4504.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 2,3-diphosphoglycerate activation Spinacia oleracea
2.7.7.27 2,3-diphosphoglycerate actvation of ADP-glucose synthesis Spinacia oleracea
2.7.7.27 3-phosphoglycerate allosteric activation Spinacia oleracea
2.7.7.27 3-phosphoglycerate activation Spinacia oleracea
2.7.7.27 3-phosphoglycerate pH-dependent Spinacia oleracea
2.7.7.27 D-fructose 1,6-bisphosphate kinetics Spinacia oleracea
2.7.7.27 D-fructose 1,6-bisphosphate activation of pyrophosphorolysis Spinacia oleracea
2.7.7.27 D-fructose 1,6-bisphosphate activation of ADP-glucose synthesis Spinacia oleracea
2.7.7.27 D-fructose 6-phosphate activation Spinacia oleracea
2.7.7.27 D-fructose 6-phosphate activation of ADPglucose synthesis Spinacia oleracea
2.7.7.27 D-ribose 5-phosphate activation Spinacia oleracea
2.7.7.27 D-ribose 5-phosphate slight activation Spinacia oleracea
2.7.7.27 D-ribose 5-phosphate activation of ADP-glucose synthesis Spinacia oleracea
2.7.7.27 phosphoenolpyruvate activation Spinacia oleracea
2.7.7.27 phosphoenolpyruvate activation of pyrophosphorolysis Spinacia oleracea
2.7.7.27 phosphoenolpyruvate ADPglucose synthesis Spinacia oleracea
2.7.7.27 Phosphoglycolate slight activation, ADP-glucose synthesis Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 ADP
-
 Spinacia oleracea
2.7.7.27 phosphate 3-phosphoglycerate reverses, increasing concentration of Mg2+ does not overcome the inhibition; kinetics Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.27 soluble
-
 Spinacia oleracea
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Spinacia oleracea one of the main regulatory steps in starch biosynthesis in plants diphosphate + ADP-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 24
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Spinacia oleracea diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate one of the main regulatory steps in starch biosynthesis in plants Spinacia oleracea diphosphate + ADP-glucose
-
 r

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.038
-
 phosphate pH 8.5, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
2.7.7.27 0.06
-
 phosphate pH 7.5, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
2.7.7.27 0.14
-
 phosphate pH 7.0, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
2.7.7.27 0.72
-
 phosphate pH 8.5, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea
2.7.7.27 1.15
-
 phosphate pH 7.5, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea
2.7.7.27 1.2
-
 ADP pH 7.5, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
2.7.7.27 1.5
-
 phosphate pH 7.0, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea
2.7.7.27 2
-
 ADP pH 7.5, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea