BRENDA - Enzyme Database

Pyrophphosphorylases in Solanum tuberosum, II. Catalytic properties an reguation of ADP-glucose and UDP-glucose pyrophosphorylase activities in potatoes

Sowokinos, J.R.; Plant Physiol. 68, 924-929 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.9
additional information
no activation by 3-phosphoglycerate
Solanum tuberosum
2.7.7.27
2,3-diphosphoglycerate
no activation
Solanum tuberosum
2.7.7.27
2-deoxy-D-ribose 5-phosphate
activation, pyrophosphorolysis
Solanum tuberosum
2.7.7.27
2-mercaptoethanol
activation, at high concentration
Solanum tuberosum
2.7.7.27
2-phospho-D-glycerate
activation; pyrophosphorolysis; slight activation
Solanum tuberosum
2.7.7.27
3-phosphoglycerate
activation; allosteric activation
Solanum tuberosum
2.7.7.27
alpha-glycerol phosphate
-
Solanum tuberosum
2.7.7.27
cysteine
slight activation, at high concentration
Solanum tuberosum
2.7.7.27
D-fructose 1,6-bisphosphate
activation of pyrophosphorolysis; slight activation
Solanum tuberosum
2.7.7.27
D-fructose 6-phosphate
activation; activation of pyrophosphorolysis
Solanum tuberosum
2.7.7.27
D-ribose 5-phosphate
activation; activation of pyrophosphorolysis
Solanum tuberosum
2.7.7.27
DTT
activation; kinetics; only slight activation in the presence of 3-phosphoglycerate
Solanum tuberosum
2.7.7.27
GSH
activation, at high concentration
Solanum tuberosum
2.7.7.27
additional information
no activation of pyrophosphorolysis; not: L-lactate, citrate; not: oxaloacetate, L-malate
Solanum tuberosum
2.7.7.27
phosphoenolpyruvate
activation; activation of pyrophosphorolysis
Solanum tuberosum
2.7.7.27
pyruvate
not
Solanum tuberosum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.27
ADP
-
Solanum tuberosum
2.7.7.27
AMP
not
Solanum tuberosum
2.7.7.27
NADP+
-
Solanum tuberosum
2.7.7.27
phosphate
strong
Solanum tuberosum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.27
additional information
-
additional information
kinetic study
Solanum tuberosum
2.7.7.27
0.24
-
ADP-glucose
-
Solanum tuberosum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.9
Mg2+
requirement
Solanum tuberosum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.9
Solanum tuberosum
-
cv. Norchip
-
2.7.7.27
Solanum tuberosum
-
potato, var. Norchip
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.9
partial
Solanum tuberosum
2.7.7.27
partial
Solanum tuberosum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.9
tuber
immature
Solanum tuberosum
-
2.7.7.27
tuber
growing
Solanum tuberosum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.9
UTP + alpha-D-glucose 1-phosphate
-
643116
Solanum tuberosum
diphosphate + UDP-glucose
-
643116
Solanum tuberosum
r
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643116
Solanum tuberosum
diphosphate + ADP-glucose
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.27
8
-
pyrophosphorolysis
Solanum tuberosum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.27
6.8
9.5
about half-maximal activity at pH 6.8 and about 70% of maximal activity at pH 9.5
Solanum tuberosum
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.27
0.088
-
phosphate
pH 7.5, 37°C, in absence of 3-phosphoglycerate
Solanum tuberosum
2.7.7.27
0.12
-
phosphate
pH 7.5, 37°C, in presence of 3-phosphoglycerate
Solanum tuberosum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.9
additional information
no activation by 3-phosphoglycerate
Solanum tuberosum
2.7.7.27
2,3-diphosphoglycerate
no activation
Solanum tuberosum
2.7.7.27
2-deoxy-D-ribose 5-phosphate
activation, pyrophosphorolysis
Solanum tuberosum
2.7.7.27
2-mercaptoethanol
activation, at high concentration
Solanum tuberosum
2.7.7.27
2-phospho-D-glycerate
activation; pyrophosphorolysis; slight activation
Solanum tuberosum
2.7.7.27
3-phosphoglycerate
activation; allosteric activation
Solanum tuberosum
2.7.7.27
alpha-glycerol phosphate
-
Solanum tuberosum
2.7.7.27
cysteine
slight activation, at high concentration
Solanum tuberosum
2.7.7.27
D-fructose 1,6-bisphosphate
activation of pyrophosphorolysis; slight activation
Solanum tuberosum
2.7.7.27
D-fructose 6-phosphate
activation; activation of pyrophosphorolysis
Solanum tuberosum
2.7.7.27
D-ribose 5-phosphate
activation; activation of pyrophosphorolysis
Solanum tuberosum
2.7.7.27
DTT
activation; kinetics; only slight activation in the presence of 3-phosphoglycerate
Solanum tuberosum
2.7.7.27
GSH
activation, at high concentration
Solanum tuberosum
2.7.7.27
additional information
no activation of pyrophosphorolysis; not: L-lactate, citrate; not: oxaloacetate, L-malate
Solanum tuberosum
2.7.7.27
phosphoenolpyruvate
activation; activation of pyrophosphorolysis
Solanum tuberosum
2.7.7.27
pyruvate
not
Solanum tuberosum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.27
ADP
-
Solanum tuberosum
2.7.7.27
AMP
not
Solanum tuberosum
2.7.7.27
NADP+
-
Solanum tuberosum
2.7.7.27
phosphate
strong
Solanum tuberosum
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.27
0.088
-
phosphate
pH 7.5, 37°C, in absence of 3-phosphoglycerate
Solanum tuberosum
2.7.7.27
0.12
-
phosphate
pH 7.5, 37°C, in presence of 3-phosphoglycerate
Solanum tuberosum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.27
additional information
-
additional information
kinetic study
Solanum tuberosum
2.7.7.27
0.24
-
ADP-glucose
-
Solanum tuberosum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.9
Mg2+
requirement
Solanum tuberosum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.9
partial
Solanum tuberosum
2.7.7.27
partial
Solanum tuberosum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.9
tuber
immature
Solanum tuberosum
-
2.7.7.27
tuber
growing
Solanum tuberosum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.9
UTP + alpha-D-glucose 1-phosphate
-
643116
Solanum tuberosum
diphosphate + UDP-glucose
-
643116
Solanum tuberosum
r
2.7.7.27
ATP + alpha-D-glucose 1-phosphate
-
643116
Solanum tuberosum
diphosphate + ADP-glucose
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.27
8
-
pyrophosphorolysis
Solanum tuberosum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.27
6.8
9.5
about half-maximal activity at pH 6.8 and about 70% of maximal activity at pH 9.5
Solanum tuberosum