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Literature summary extracted from

  • Sowokinos, J.R.
    Pyrophphosphorylases in Solanum tuberosum, II. Catalytic properties an reguation of ADP-glucose and UDP-glucose pyrophosphorylase activities in potatoes (1981), Plant Physiol., 68, 924-929.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.9 additional information no activation by 3-phosphoglycerate Solanum tuberosum
2.7.7.27 2,3-diphosphoglycerate no activation Solanum tuberosum
2.7.7.27 2-deoxy-D-ribose 5-phosphate activation, pyrophosphorolysis Solanum tuberosum
2.7.7.27 2-mercaptoethanol activation, at high concentration Solanum tuberosum
2.7.7.27 2-phospho-D-glycerate activation Solanum tuberosum
2.7.7.27 2-phospho-D-glycerate slight activation Solanum tuberosum
2.7.7.27 2-phospho-D-glycerate pyrophosphorolysis Solanum tuberosum
2.7.7.27 3-phosphoglycerate allosteric activation Solanum tuberosum
2.7.7.27 3-phosphoglycerate activation Solanum tuberosum
2.7.7.27 alpha-glycerol phosphate
-
 Solanum tuberosum
2.7.7.27 cysteine slight activation, at high concentration Solanum tuberosum
2.7.7.27 D-fructose 1,6-bisphosphate slight activation Solanum tuberosum
2.7.7.27 D-fructose 1,6-bisphosphate activation of pyrophosphorolysis Solanum tuberosum
2.7.7.27 D-fructose 6-phosphate activation Solanum tuberosum
2.7.7.27 D-fructose 6-phosphate activation of pyrophosphorolysis Solanum tuberosum
2.7.7.27 D-ribose 5-phosphate activation Solanum tuberosum
2.7.7.27 D-ribose 5-phosphate activation of pyrophosphorolysis Solanum tuberosum
2.7.7.27 DTT activation Solanum tuberosum
2.7.7.27 DTT kinetics Solanum tuberosum
2.7.7.27 DTT only slight activation in the presence of 3-phosphoglycerate Solanum tuberosum
2.7.7.27 GSH activation, at high concentration Solanum tuberosum
2.7.7.27 additional information not: L-lactate, citrate Solanum tuberosum
2.7.7.27 additional information no activation of pyrophosphorolysis Solanum tuberosum
2.7.7.27 additional information not: oxaloacetate, L-malate Solanum tuberosum
2.7.7.27 phosphoenolpyruvate activation Solanum tuberosum
2.7.7.27 phosphoenolpyruvate activation of pyrophosphorolysis Solanum tuberosum
2.7.7.27 pyruvate not Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 ADP
-
 Solanum tuberosum
2.7.7.27 AMP not Solanum tuberosum
2.7.7.27 NADP+
-
 Solanum tuberosum
2.7.7.27 phosphate strong Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 additional information
-
 additional information kinetic study Solanum tuberosum
2.7.7.27 0.24
-
 ADP-glucose
-
 Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.9 Mg2+ requirement Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Solanum tuberosum
-
 cv. Norchip
-
2.7.7.27 Solanum tuberosum
-
 potato, var. Norchip
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9 partial Solanum tuberosum
2.7.7.27 partial Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.9 tuber immature Solanum tuberosum
-
2.7.7.27 tuber growing Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + UDP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + ADP-glucose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.27 8
-
 pyrophosphorolysis Solanum tuberosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.27 6.8 9.5 about half-maximal activity at pH 6.8 and about 70% of maximal activity at pH 9.5 Solanum tuberosum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.088
-
 phosphate pH 7.5, 37°C, in absence of 3-phosphoglycerate Solanum tuberosum
2.7.7.27 0.12
-
 phosphate pH 7.5, 37°C, in presence of 3-phosphoglycerate Solanum tuberosum