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Literature summary extracted from
- The enzymatic synthesis of thymidine-linked sugars I. Thymidine diphosphate glucose (1961), J. Biol. Chem., 236, 1791-1794.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.7.24 | 0.05 | - |
TDP-glucose | 25°C, pH 8 | Pseudomonas aeruginosa |  |
| 2.7.7.24 | 0.1 | - |
TTP | 25°C, pH 8 | Pseudomonas aeruginosa | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.7.24 | Mg2+ | absolute requirement, 2 mM: optimal | Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.7.24 | dTTP + alpha-D-glucose 1-phosphate | Pseudomonas aeruginosa | - |
dTDP-glucose + diphosphate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.7.24 | Pseudomonas aeruginosa | - |
ATCC 7700 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.7.24 | ATCC 7700, partial | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.7.24 | 44.8 | - |
dTDPglucose + diphosphate | Pseudomonas aeruginosa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.7.7.24 | stable for several weeks at any step of purification if kept frozen | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.7.24 | dTTP + alpha-D-glucose 1-phosphate | - |
Pseudomonas aeruginosa | dTDP-glucose + diphosphate | - |
r | |
| 2.7.7.24 | TTP + alpha-D-glucose 1-phosphate | - |
Pseudomonas aeruginosa | TDP-glucose + diphosphate | - |
r | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.7.24 | 25 | - |
assay at, dTDP-glucose + diphosphate | Pseudomonas aeruginosa |
| 2.7.7.24 | 37 | - |
assay at, dTTP + alpha-D-glucose 1-phosphate | Pseudomonas aeruginosa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.7.24 | 8 | - |
broad | Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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