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Literature summary extracted from

  • Sulzenbacher, G.; Gal, L.; Peneff, C.; Fassy, F.; Bourne, Y.
    Crystal structure of Streptococcus pneumoniae N-acetylglucosamine-1-phosphate uridyltransferase bound to acetyl-coenzyme A reveals a novel active site architecture (2001), J. Biol. Chem., 276, 11844-11851.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.23 medicine potential target for antibacterial agents Streptococcus pneumoniae
2.7.7.23 medicine potential target for antibacterial agents Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.23 expressed in M15 cells Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.157 hanging-drop vapour-diffusion method. Crystal structures of the enzyme in unbound form, in complex with acetyl-coenzyme A and in complex with both AcCoA and the end product UDP-GlcNAc, determined and refined to 2.3, 2.5, and 1.75 A, respectively. GlmU molecule is organized in two separate domains connected via a long alpha-helical linker and associates as a trimer, with the 50-A-long left-handed beta-helix (LbH) C-terminal domains packed against each other in a parallel fashion and the C-terminal region extended far away from the LbH core and exchanged with the beta-helix from a neighboring subunit in the trimer. AcCoA binding induces the formation of a long and narrow tunnel, enclosed between two adjacent LbH domains and the interchanged C-terminal region of the third subunit, giving rise to an original active site architecture at the junction of three subunits Streptococcus pneumoniae
2.7.7.23 recombinant enzyme, hanging drop vapor diffusion method Streptococcus pneumoniae
2.7.7.23 recombinant enzyme, hanging drop vapor diffusion method Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.157 D-glucosamine 1-phosphate + acetyl-CoA Streptococcus pneumoniae the bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase and performs the last two steps in the synthesis of UDP-N-acetylglucosamine, which is an essential precursor in bacterial cell wall biosynthesis N-acetyl-D-glucosamine 1-phosphate + CoA
-
?
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Streptococcus pneumoniae amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Escherichia coli amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Streptococcus pneumoniae involved in synthesis of peptidoglycan and lipopolysaccharide UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Escherichia coli involved in synthesis of peptidoglycan and lipopolysaccharide UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.157 Streptococcus pneumoniae Q97R46
-
-
2.7.7.23 Escherichia coli P0ACC7
-
-
2.7.7.23 Streptococcus pneumoniae Q97R46
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.23 recombinant enzyme Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.157 D-glucosamine 1-phosphate + acetyl-CoA the bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase and performs the last two steps in the synthesis of UDP-N-acetylglucosamine, which is an essential precursor in bacterial cell wall biosynthesis Streptococcus pneumoniae N-acetyl-D-glucosamine 1-phosphate + CoA
-
?
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine
-
Streptococcus pneumoniae UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine
-
Escherichia coli UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Streptococcus pneumoniae UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Escherichia coli UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine involved in synthesis of peptidoglycan and lipopolysaccharide Streptococcus pneumoniae UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine involved in synthesis of peptidoglycan and lipopolysaccharide Escherichia coli UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r

Subunits

EC Number Subunits Comment Organism
2.7.7.23 trimer
-
Escherichia coli
2.7.7.23 trimer crystal structure analysis Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
2.3.1.157 GlmU bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase Streptococcus pneumoniae
2.3.1.157 N-acetylglucosamine-1-phosphate uridyltransferase bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase Streptococcus pneumoniae
2.7.7.23 More bifunctional enzyme with activity of: glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase Streptococcus pneumoniae
2.7.7.23 More bifunctional enzyme with activity of: glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase Escherichia coli