Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamamoto, K.; Moriguchi, M.; Kawai, H.; Tochikura, T.
    Purification and some properties of uridine diphosphate N-acetylglucosamine pyrophosphorylase from Neurospora crassa (1979), Can. J. Microbiol., 25, 1381-1386.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.23 2-mercaptoethanol slight stimulation Neurospora crassa
2.7.7.23 dithioerythritol highly stimulating Neurospora crassa
2.7.7.23 dithiothreitol highly stimulating Neurospora crassa
2.7.7.23 glutathione slight stimulation Neurospora crassa

General Stability

EC Number General Stability Organism
2.7.7.23 dithiothreitol stabilizes, activity lost during storage can be recovered by addition of dithiothreitol Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.23 2.2
-
 UDP-N-acetyl-D-glucosamine pH 7.5, 30°C Neurospora crassa
2.7.7.23 5.4
-
 diphosphate pH 7.5, 30°C Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.23 Co2+ can partially replace Mg2+ Neurospora crassa
2.7.7.23 Mg2+ required for maximum activity Neurospora crassa
2.7.7.23 Mn2+ can partially replace Mg2+ Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.23 37000
-
 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Neurospora crassa amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Neurospora crassa involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Neurospora crassa IFO 6178 amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Neurospora crassa IFO 6178 involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.23 Neurospora crassa
-
-
-
2.7.7.23 Neurospora crassa IFO 6178
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.23
-
 Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.23 8.66
-
 purified enzyme, pH 7.5, 30°C Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
2.7.7.23 -20°C, phosphate buffer, pH 7.5, stable for at least 2 weeks Neurospora crassa
2.7.7.23 4°C, phosphate buffer, pH 7.5, 0.01 mM dithiothreitol, stable for at least 2 weeks Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine highly specific, no activity with any other sugar nucloetide tested Neurospora crassa UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Neurospora crassa UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin Neurospora crassa UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine highly specific, no activity with any other sugar nucloetide tested Neurospora crassa IFO 6178 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Neurospora crassa IFO 6178 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin Neurospora crassa IFO 6178 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.23 40
-
 5 min, 50% of activity lost in the absence of dithiothreitol, 20% of activity lost in presence of dithiothreitol Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.23 7.5
-
-
 Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.23 5 9
-
 Neurospora crassa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.23 6 7.5 most stable Neurospora crassa