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Literature summary extracted from

  • Strominger, J.L.; Smith, M.S.
    Uridine diphosphoacetylglucosamine pyrophosphorylase (1959), J. Biol. Chem., 234, 1822-1827.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.23 labile toward fractionation with ammonium sulfate, inactivation can not be reversed by dialysis Bos taurus
2.7.7.23 more stable toward ammonium sulfate then calf liver enzyme Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.23 fluoride inhibits Mg2+ but not Mn2+ activated enzyme Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.23 Mg2+ required for maximum activity Staphylococcus aureus
2.7.7.23 Mg2+ required for maximum activity Bos taurus
2.7.7.23 Mn2+ can partially replace Mg2+ Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Staphylococcus aureus amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Bos taurus amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.7.23 organic solvents labile to fractionation with Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.23 Bos taurus
-
calf
-
2.7.7.23 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.23
-
Staphylococcus aureus
2.7.7.23
-
Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.23 liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.23 330
-
purified enzyme, pH 7.4, 37°C Bos taurus
2.7.7.23 2390
-
purified enzyme, pH 7.4, 37°C Staphylococcus aureus

Storage Stability

EC Number Storage Stability Organism
2.7.7.23 -20°C, acetone precipitated enzyme, 3 months, no loss of activity Bos taurus
2.7.7.23 -20°C, crude extract, 5-10% loss of activity per day of storage Bos taurus
2.7.7.23 -20°C, purified enzyme or crude extract, 2 months, 10% loss of activity Staphylococcus aureus
2.7.7.23 -20°C, purified enzyme, 10% loss of activity per week of storage Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.23 diphosphate + UDP-glucose 30% of the activity with UDP-N-acetylglucosamine Bos taurus UTP + D-glucose 1-phosphate
-
?
2.7.7.23 diphosphate + UDP-N-acetyl-D-galactosamine 2.8% of the activity with UDP-N-acetylglucosamine Staphylococcus aureus UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
?
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine
-
Staphylococcus aureus UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine
-
Bos taurus UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Staphylococcus aureus UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Bos taurus UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.23 7
-
-
Staphylococcus aureus
2.7.7.23 7
-
-
Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.23 5.5 8.5
-
Staphylococcus aureus