EC Number | General Stability | Organism |
---|---|---|
2.7.7.23 | labile toward fractionation with ammonium sulfate, inactivation can not be reversed by dialysis | Bos taurus |
2.7.7.23 | more stable toward ammonium sulfate then calf liver enzyme | Staphylococcus aureus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.23 | fluoride | inhibits Mg2+ but not Mn2+ activated enzyme | Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.23 | Mg2+ | required for maximum activity | Staphylococcus aureus | |
2.7.7.23 | Mg2+ | required for maximum activity | Bos taurus | |
2.7.7.23 | Mn2+ | can partially replace Mg2+ | Staphylococcus aureus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.23 | diphosphate + UDP-N-acetyl-D-glucosamine | Staphylococcus aureus | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
2.7.7.23 | diphosphate + UDP-N-acetyl-D-glucosamine | Bos taurus | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
2.7.7.23 | organic solvents | labile to fractionation with | Staphylococcus aureus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.23 | Bos taurus | - |
calf | - |
2.7.7.23 | Staphylococcus aureus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.23 | - |
Staphylococcus aureus |
2.7.7.23 | - |
Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.23 | liver | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.23 | 330 | - |
purified enzyme, pH 7.4, 37°C | Bos taurus |
2.7.7.23 | 2390 | - |
purified enzyme, pH 7.4, 37°C | Staphylococcus aureus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.7.23 | -20°C, acetone precipitated enzyme, 3 months, no loss of activity | Bos taurus |
2.7.7.23 | -20°C, crude extract, 5-10% loss of activity per day of storage | Bos taurus |
2.7.7.23 | -20°C, purified enzyme or crude extract, 2 months, 10% loss of activity | Staphylococcus aureus |
2.7.7.23 | -20°C, purified enzyme, 10% loss of activity per week of storage | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.23 | diphosphate + UDP-glucose | 30% of the activity with UDP-N-acetylglucosamine | Bos taurus | UTP + D-glucose 1-phosphate | - |
? | |
2.7.7.23 | diphosphate + UDP-N-acetyl-D-galactosamine | 2.8% of the activity with UDP-N-acetylglucosamine | Staphylococcus aureus | UTP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.7.23 | diphosphate + UDP-N-acetyl-D-glucosamine | - |
Staphylococcus aureus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
2.7.7.23 | diphosphate + UDP-N-acetyl-D-glucosamine | - |
Bos taurus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
2.7.7.23 | diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Staphylococcus aureus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
2.7.7.23 | diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Bos taurus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.23 | 7 | - |
- |
Staphylococcus aureus |
2.7.7.23 | 7 | - |
- |
Bos taurus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.23 | 5.5 | 8.5 | - |
Staphylococcus aureus |