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Literature summary extracted from

  • Antoniades, D.; Antonoglou, O.
    Poly(A) polymerases of rat liver nuclei. Purification and specificity (1978), Biochim. Biophys. Acta, 519, 447-460.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.19 nucleus 2 forms: one from chromatin and one from nucleoplasm Rattus norvegicus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 Mg2+ optimal concentration: 8-10 mM, polymerase I from chromatin, polymerase II from nucleoplasm is inactive in presence of Mg2+ Rattus norvegicus
2.7.7.19 Mn2+ optimal concentration: 0.8 mM (polymerase I and II) Rattus norvegicus
2.7.7.19 additional information low ionic strength required for maximal activity Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.19 ATP + RNA Rattus norvegicus 2 enzymes participate in the polyadenylation of chromosomal RNA, by a coupled mechanism, the chromatin bound enzyme adds 120-130 adenosine nucleotides to chromosomal RNA, the nucleoplasmic enzyme completes the polyadenylation by adding 80-90 more AMP units to the polyadenylated end ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19 2 forms, one from chromatin and one from nucleoplasm Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.19 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.19 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + RNA highly specific for ATP Rattus norvegicus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA chromatin enzyme uses chromosomal RNA as primer, enzyme from nucleoplasm uses poly(A) and hnRNA isolated from chromatin as primer Rattus norvegicus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA primer required Rattus norvegicus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA 2 enzymes participate in the polyadenylation of chromosomal RNA, by a coupled mechanism, the chromatin bound enzyme adds 120-130 adenosine nucleotides to chromosomal RNA, the nucleoplasmic enzyme completes the polyadenylation by adding 80-90 more AMP units to the polyadenylated end Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.19 8
-
 polymerase II (nucleoplasm) Rattus norvegicus
2.7.7.19 8.5
-
 polymerase I (chromatin) Rattus norvegicus